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Literature summary for 3.2.1.55 extracted from
- alpha-L-arabinofuranosidases from Aspergillus terreus with potential application in enology: Induction, purification, and characterization (1997), J. Agric. Food Chem., 45, 2379-2383.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| arabitol | best inducer | Aspergillus terreus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Aspergillus terreus | |
| glucose | reduces original activity of ABF2 and ABF3 by 20% | Aspergillus terreus | |
| Hg2+ | - |
Aspergillus nidulans | |
| Hg2+ | - |
Aspergillus terreus | |
| Hg2+ | - |
Pectobacterium carotovorum | |
| Hg2+ | - |
Streptomyces purpurascens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | isoenzyme ABF3 | Aspergillus terreus | |
| 0.35 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | isoenzyme ABF1 | Aspergillus terreus | |
| 0.47 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | isoenzyme ABF2 | Aspergillus terreus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus nidulans | - |
- |
| extracellular | - |
Aspergillus niger | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78500 | - |
isoenzyme ABF3 | Aspergillus terreus |
| 82000 | - |
isoenzyme ABF2 | Aspergillus terreus |
| 90000 | - |
isoenzyme ABF21 | Aspergillus terreus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
- |
- |
| Aspergillus niger | - |
- |
- |
| Aspergillus terreus | - |
CECT 2663 | - |
| Aspergillus terreus | - |
VTT-D-82209 | - |
| Aspergillus terreus CECT 2663 | - |
CECT 2663 | - |
| Aspergillus terreus VTT-D-82209 | - |
VTT-D-82209 | - |
| Bacillus subtilis | - |
- |
- |
| Pectobacterium carotovorum | - |
- |
- |
| Sclerotinia fructigena | - |
- |
- |
| Sclerotinia sclerotiorum | - |
- |
- |
| Streptomyces purpurascens | - |
- |
- |
| Trichoderma reesei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
- |
Sclerotinia sclerotiorum |
| 3 isoenzymes | Aspergillus terreus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
isoenzyme ABF3 | Aspergillus terreus |
| 50.4 | - |
isoenzyme ABF2 | Aspergillus terreus |
| 76.6 | - |
isoenzyme ABF1 | Aspergillus terreus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus terreus | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus terreus CECT 2663 | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus terreus VTT-D-82209 | p-nitrophenol + L-arabinose | - |
? | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus nidulans | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Pectobacterium carotovorum | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus terreus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Sclerotinia sclerotiorum | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Sclerotinia fructigena | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus terreus CECT 2663 | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus terreus VTT-D-82209 | L-arabinose + phenol | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus niger |
| 50 | - |
isoenzyme ABF1 | Aspergillus terreus |
| 60 | - |
- |
Bacillus subtilis |
| 60 | - |
- |
Aspergillus niger |
| 60 | - |
- |
Trichoderma reesei |
| 60 | - |
isoenzyme ABF1 | Aspergillus terreus |
| 65 | - |
- |
Aspergillus nidulans |
| 65 | - |
isoenzyme ABF3 | Aspergillus terreus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | 75 | - |
Aspergillus terreus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Aspergillus nidulans |
| 60 | - |
isoenzymes ABF1 and ABF3 seemed to be quite stable | Aspergillus terreus |
| 65 | - |
isoenzyme ABF2 retains 7% activity after 4 h | Aspergillus terreus |
| 70 | - |
isoenzymes ABF1 and ABF3, half-life 2 h and 5 h | Aspergillus terreus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus niger |
| 5 | - |
isoenzyme ABF1 and ABF2 | Aspergillus terreus |
| 5.5 | - |
- |
Aspergillus nidulans |
| 5.5 | - |
isoenzyme ABF3 | Aspergillus terreus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 4.4 | - |
Aspergillus terreus |
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