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Literature summary for 3.2.1.55 extracted from
- Debranching of arabinoxylan: Properties of the thermoactive recombinant alpha-L-arabinofuranosidase from Clostridium stercoarium (ArfB) (1995), Appl. Microbiol. Biotechnol., 43, 856-860.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | - |
Aspergillus niger |
| analysis | enzymatic tool for structural analysis of oligo- and polysaccharides | Thermoclostridium stercorarium |
| degradation | application potential for industrial purposes, biomass degradation and refining, extremely low end-product inhibition by arabinose further increases its applicability | Thermoclostridium stercorarium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene, encoding AF, heterologously expressed in Escherichia coli JM83 | Thermoclostridium stercorarium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Thermoclostridium stercorarium |  |
| ethanol | slight inhibition | Thermoclostridium stercorarium | |
| Hg2+ | - |
Geobacillus stearothermophilus | |
| Hg2+ | - |
Streptomyces purpurascens | |
| Hg2+ | - |
Thermoclostridium stercorarium | |
| p-hydroxymercuribenzoate | - |
Thermoclostridium stercorarium | |
| SDS | - |
Thermoclostridium stercorarium | |
| Zn2+ | - |
Thermoclostridium stercorarium | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Geobacillus stearothermophilus | - |
- |
| extracellular | - |
Aspergillus niger | - |
- |
| extracellular | - |
Ruminococcus albus | - |
- |
| extracellular | - |
Thermoclostridium stercorarium | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
zymogram staining | Thermoclostridium stercorarium |
| 75000 | - |
4 * 75000, SDS-PAGE | Ruminococcus albus |
| 195000 | - |
gel filtration | Thermoclostridium stercorarium |
| 310000 | - |
- |
Ruminococcus albus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oat spelt xylan + H2O | Geobacillus stearothermophilus | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Butyrivibrio fibrisolvens | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Thermoclostridium stercorarium | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Butyrivibrio fibrisolvens GS113 | - |
L-arabinose + ? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | resistance to ethanol, neutral detergents and NaCl | Thermoclostridium stercorarium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Butyrivibrio fibrisolvens | - |
GS113 | - |
| Butyrivibrio fibrisolvens GS113 | - |
GS113 | - |
| Geobacillus stearothermophilus | - |
- |
- |
| Ruminococcus albus | - |
8 | - |
| Ruminococcus albus 8 | - |
8 | - |
| Streptomyces purpurascens | - |
- |
- |
| Thermoclostridium stercorarium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ruminococcus albus |
| recombinant protein, arfB gene product | Thermoclostridium stercorarium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 883 | - |
recombinant arfB gene product | Thermoclostridium stercorarium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | 0.26% of activity observed with p-nitrophenyl-alpha-L-arabinofuranoside | Thermoclostridium stercorarium | 4-nitrophenol + D-glucopyranose | - |
? | |
| alkyl-alpha-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | L-arabinose + alkyl alcohol | - |
? | |
| aryl-alpha-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | L-arabinose + aryl alcohol | - |
? | |
| L-arabinogalactan + H2O | - |
Geobacillus stearothermophilus | L-arabinose + ? | - |
? | |
| L-arabinogalactan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
| L-arabinogalactan + H2O | - |
Thermoclostridium stercorarium | L-arabinose + ? | - |
? | |
| L-arabinoxylan + H2O | - |
Geobacillus stearothermophilus | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Thermoclostridium stercorarium | L-arabinose + ? | - |
ir | |
| methylumbelliferyl-alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens | methylumbelliferone | - |
? | |
| methylumbelliferyl-alpha-L-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | methylumbelliferone | - |
? | |
| methylumbelliferyl-alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens GS113 | methylumbelliferone | - |
? | |
| oat spelt xylan + H2O | - |
Geobacillus stearothermophilus | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Butyrivibrio fibrisolvens | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Thermoclostridium stercorarium | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Butyrivibrio fibrisolvens GS113 | L-arabinose + ? | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus 8 | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-beta-D-galactopyranoside + H2O | 0.33% of activity observed with p-nitrophenyl-alpha-L-arabinofuranoside | Thermoclostridium stercorarium | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
| p-nitrophenyl-beta-D-xylopyranoside + H2O | 0.44% of activity observed with p-nitrophenyl-alpha-L-arabinofuranoside | Thermoclostridium stercorarium | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus 8 | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens GS113 | L-arabinose + phenol | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Geobacillus stearothermophilus |
| tetramer | 4 * 52000, SDS-PAGE | Thermoclostridium stercorarium |
| tetramer | 4 * 75000, SDS-PAGE | Ruminococcus albus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Geobacillus stearothermophilus |
| 70 | - |
- |
Thermoclostridium stercorarium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 90 | - |
Thermoclostridium stercorarium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 90 | remarkably thermostable, half of its activity remains after 0.7 h at 90°C | Thermoclostridium stercorarium |
| 85 | - |
recombinant arfB gene product, stable up to | Thermoclostridium stercorarium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus niger |
| 5 | - |
- |
Thermoclostridium stercorarium |
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