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Literature summary for 3.2.1.55 extracted from

  • Kimura, I.; Sasahara, H.; Tajima, S.
    Purification and characterization of two xylanases and an arabinofuranosidase from Aspergillus sojae (1995), J. Ferment. Bioeng., 80, 334-339.
No PubMed abstract available

Application

Application Comment Organism
nutrition
-
Aspergillus niger
nutrition most commonly used in soy sauce manufacture for producing koji mold Aspergillus sojae

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Aspergillus sojae
Mn2+
-
Aspergillus sojae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
gel filtration Aspergillus sojae
34300
-
SDS-PAGE Aspergillus sojae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oat spelt xylan + H2O Aspergillus niger
-
L-arabinose + ?
-
?
oat spelt xylan + H2O Aspergillus sojae
-
L-arabinose + ?
-
?
wheat bran + H2O Aspergillus sojae
-
L-arabinose + ?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus sojae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger
-
Aspergillus sojae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
69.5
-
-
Aspergillus sojae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arabinan + H2O
-
Aspergillus niger (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Aspergillus sojae (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinoxylan + H2O
-
Aspergillus niger L-arabinose + ?
-
ir
L-arabinoxylan + H2O
-
Aspergillus sojae L-arabinose + ?
-
ir
additional information
-
Aspergillus niger ?
-
?
additional information enzyme cannot hydrolyze p-nitrophenyl beta-D-galactopyranoside Aspergillus sojae ?
-
?
oat spelt xylan + H2O
-
Aspergillus niger L-arabinose + ?
-
?
oat spelt xylan + H2O
-
Aspergillus sojae L-arabinose + ?
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Aspergillus niger p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Aspergillus sojae p-nitrophenol + L-arabinose
-
?
wheat bran + H2O
-
Aspergillus sojae L-arabinose + ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Aspergillus niger
monomer 1 * 34300, SDS-PAGE Aspergillus sojae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Aspergillus niger
50
-
-
Aspergillus sojae
60
-
-
Aspergillus niger
60
-
-
Aspergillus sojae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable up to Aspergillus sojae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus niger
5
-
-
Aspergillus sojae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9
-
Aspergillus sojae