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Literature summary for 3.2.1.55 extracted from
- alpha-L-arabinofuranosidase production by Aspergillus nidulans (1994), FEMS Microbiol. Lett., 115, 107-112.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| lactose | induces enzyme activity | Aspergillus nidulans |  |
| xylose | induces enzyme activity | Aspergillus nidulans | |
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | enzyme of industrial interest because of its ability to improve hydrolytic action of other glycanases on hemicellulolytic polysaccharides | Aspergillus nidulans |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Aspergillus nidulans | |
| Hg2+ | - |
Streptomyces purpurascens | |
| Hg2+ | - |
Streptomyces sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.48 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Aspergillus niger | |
| 0.6 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Aspergillus niger | |
| 1.2 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Trichoderma reesei | |
| 1.64 | - |
4-nitrophenyl alpha-L-arabinofuranoside | - |
Dichomitus squalens | |
| 2.7 | - |
4-nitrophenyl alpha-L-arabinofuranoside | - |
Aspergillus nidulans | |
| 4.1 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Aspergillus niger | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus nidulans | - |
- |
| extracellular | - |
Aspergillus niger | - |
- |
| extracellular | - |
Ruminococcus albus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
SDS-PAGE | Aspergillus nidulans |
| 53000 | - |
- |
Trichoderma reesei |
| 53000 | - |
strain KI | Aspergillus niger |
| 60000 | - |
- |
Aspergillus niger |
| 60000 | - |
- |
Dichomitus squalens |
| 128000 | - |
- |
Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beet arabinan + H2O | Aspergillus niger | extracted from beet pulp | L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | Aspergillus niger KI | extracted from beet pulp | L-arabinose + ? | - |
ir | |
| birchwood xylan + H2O | Aspergillus nidulans | - |
L-arabinose + ? | - |
? | |
| gum arabic + H2O | Aspergillus nidulans | - |
L-arabinose + ? | - |
? | |
| gum arabic + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
? | |
| gum arabic + H2O | Aspergillus niger KI | - |
L-arabinose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
- |
- |
| Aspergillus niger | - |
- |
- |
| Aspergillus niger KI | - |
- |
- |
| Dichomitus squalens | - |
- |
- |
| Ruminococcus albus | - |
8 | - |
| Ruminococcus albus 8 | - |
8 | - |
| Streptomyces purpurascens | - |
IFO 3389 | - |
| Streptomyces sp. | - |
- |
- |
| Trichoderma reesei | - |
filamentous fungus, VTT-D-79125 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
- |
Ruminococcus albus |
- |
Dichomitus squalens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus nidulans | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Dichomitus squalens | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| alpha-L-arabinofuranosyl 4-methylumbelliferone + H2O | - |
Aspergillus nidulans | methylumbelliferone + alpha-L-arabinofuranose | - |
? | |
| beet arabinan + H2O | extracted from beet pulp | Aspergillus niger | L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | extracted from beet pulp | Aspergillus niger KI | L-arabinose + ? | - |
ir | |
| birchwood xylan + H2O | - |
Aspergillus nidulans | L-arabinose + ? | - |
? | |
| gum arabic + H2O | - |
Aspergillus nidulans | L-arabinose + ? | - |
? | |
| gum arabic + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
| gum arabic + H2O | - |
Aspergillus niger KI | L-arabinose + ? | - |
? | |
| L-arabinogalactan + H2O | - |
Aspergillus nidulans | L-arabinose + ? | - |
? | |
| L-arabinogalactan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
| L-arabinogalactan + H2O | - |
Streptomyces sp. | L-arabinose + ? | - |
? | |
| L-arabinogalactan + H2O | - |
Dichomitus squalens | L-arabinose + ? | - |
? | |
| L-arabinogalactan + H2O | - |
Aspergillus niger KI | L-arabinose + ? | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger KI | p-nitrophenol + L-arabinose | - |
? | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus nidulans | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Dichomitus squalens | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus 8 | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger KI | L-arabinose + phenol | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Aspergillus nidulans |
| 50 | - |
- |
Aspergillus niger |
| 55 | - |
- |
Aspergillus niger |
| 55 | - |
crude enzyme preparation | Aspergillus nidulans |
| 60 | - |
- |
Aspergillus niger |
| 60 | - |
- |
Trichoderma reesei |
| 60 | - |
- |
Dichomitus squalens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life time 1.5 h, inactivation after 2 h at 70°C | Aspergillus nidulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Dichomitus squalens |
| 3.7 | - |
- |
Aspergillus niger |
| 3.8 | 4 | towards phenyl alpha-L-arabinofuranoside or beet arabinan | Aspergillus niger |
| 4 | - |
- |
Aspergillus niger |
| 4 | - |
- |
Trichoderma reesei |
| 4.1 | - |
- |
Aspergillus niger |
| 4.5 | 5.5 | - |
Aspergillus niger |
| 5.5 | - |
crude enzyme preparation | Aspergillus nidulans |
| 6 | 6.5 | - |
Aspergillus niger |
| 7 | - |
- |
Aspergillus nidulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 5.5 | unstable above | Aspergillus nidulans |
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