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Literature summary for 3.2.1.55 extracted from

  • Hirano, Y.; Tsumuraya, Y.; Hashimoto, Y.
    Characterization of spinach leaf alpha-L-arabinofuranosidases and beta-galactosidases and their synergistic action on an endogenous arabinogalactan-protein (1994), Physiol. Plant., 92, 286-296.
No PubMed abstract available
Search for more literature for 3.2.1.55

Inhibitors

Inhibitors Comment Organism Structure
Ag+ alpha-L-arafase I Spinacia oleracea
D-glucono-1,5-lactone alpha-L-arafase I Spinacia oleracea
D-xylose alpha-arafase I Spinacia oleracea
Hg2+ alpha-L-arafase I Spinacia oleracea
SDS alpha-L-arafase I Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 p-nitrophenyl-alpha-L-arabinofuranoside alpha-L-arafase I Spinacia oleracea
2.2
-
 p-nitrophenyl-alpha-L-arabinofuranoside alpha-L-arafase II Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
cell membrane
-
 Spinacia oleracea
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 alpha-L-arafase II Spinacia oleracea
118000
-
 alpha-L-arafase I Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sugar beet arabinan + H2O Spinacia oleracea alpha-L-arafase II L-arabinose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Raphanus sativus
-
 radish
-
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2 isoenzymes, alpha-L-arafase I and alpha-L-arafase II Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.61
-
 alpha-L-arafase I Spinacia oleracea
2.5
-
 alpha-L-arafase II Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Spinacia oleracea p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-beta-D-xylopyranoside + H2O alpha-L-arafase I Spinacia oleracea 4-nitrophenol + beta-D-xylopyranose
-
 ?
sugar beet arabinan + H2O alpha-L-arafase II Spinacia oleracea L-arabinose + ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha-L-arafase I
-
 Spinacia oleracea
alpha-L-arafase II
-
 Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
 Spinacia oleracea