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Literature summary for 3.2.1.55 extracted from
- Purification and mechanistic properties of an extracellular alpha-L-arabinofuranosidase from Monilinia fructigena (1987), Biochem. J., 245, 843-849.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-alpha-L-arabinofuranosylmethyl-3-p-nitrophenyltriazene | - |
Monilinia fructigena |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
alpha-L-arabinofuranosyl 4-phenylphenol | - |
Monilinia fructigena | |
| 0.22 | - |
alpha-L-arabinofuranosyl 1-naphthol | - |
Monilinia fructigena | |
| 0.48 | - |
alpha-L-arabinofuranosyl 4-methylumbelliferone | - |
Monilinia fructigena | |
| 0.71 | - |
alpha-L-arabinofuranosyl 3,4-dinitrophenol | - |
Monilinia fructigena | |
| 0.82 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Monilinia fructigena | |
| 0.83 | - |
alpha-L-arabinofuranosyl 3,5-dinitrophenol | - |
Monilinia fructigena | |
| 4.1 | - |
alpha-L-arabinofuranosyl 4-bromoisoquinoline | - |
Monilinia fructigena | |
| 4.6 | - |
alpha-L-arabinofuranosyl 4-phenylphenol | - |
Monilinia fructigena | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
SDS-PAGE | Monilinia fructigena |
| 35000 | - |
SDS-PAGE | Monilinia fructigena |
| 40000 | - |
gel filtration | Monilinia fructigena |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Monilinia fructigena | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzyme designated AFIII | Monilinia fructigena |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Monilinia fructigena |
| 9.4 | - |
isoenzyme AFIII | Monilinia fructigena |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-L-arabinofuranosyl 1-naphthol + H2O | - |
Monilinia fructigena | alpha-L-arabinofuranose + 1-naphthol | - |
? | |
| alpha-L-arabinofuranosyl 3,4-dinitrophenol + H2O | - |
Monilinia fructigena | alpha-L-arabinofuranose + 3,4-dinitrophenol | - |
? | |
| alpha-L-arabinofuranosyl 3,5-dinitrophenol + H2O | - |
Monilinia fructigena | alpha-L-arabinofuranose + 3,5-dinitrophenol | - |
? | |
| alpha-L-arabinofuranosyl 4-bromoisoquinoline + H2O | - |
Monilinia fructigena | alpha-L-arabinofuranose + 4-bromoisoquinoline | - |
? | |
| alpha-L-arabinofuranosyl 4-methylumbelliferone + H2O | - |
Monilinia fructigena | methylumbelliferone + alpha-L-arabinofuranose | - |
? | |
| alpha-L-arabinofuranosyl 4-phenylphenol + H2O | - |
Monilinia fructigena | alpha-L-arabinofuranose + 4-phenylphenol | - |
? | |
| p-nitrophenyl L-arabinoside + H2O | - |
Monilinia fructigena | L-arabinose + 4-nitrophenol | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Monilinia fructigena | p-nitrophenol + L-arabinose | - |
? | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Monilinia fructigena | L-arabinose + phenol | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Monilinia fructigena |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.1 | - |
isozyme high molecular weight form | Monilinia fructigena |
| 5 | - |
AFIII, isozyme low-MW form | Monilinia fructigena |
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