Application | Comment | Organism |
---|---|---|
nutrition | food industry, preparation of wines and fruit juices | Bacillus subtilis |
nutrition | food industry, preparation of wines and fruit juices | Aspergillus niger |
nutrition | food industry, preparation of wines and fruit juices | Athelia rolfsii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mercuric acetate | - |
Aspergillus niger | |
Sodium tetrathionate | - |
Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
L-arabinan | - |
Aspergillus niger | |
2 | - |
(1-5)-L-arabinan | - |
Aspergillus niger | |
2.83 | - |
phenyl alpha-L-arabinofuranoside | - |
Athelia rolfsii | |
4.7 | - |
phenyl alpha-L-arabinofuranoside | - |
Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
- |
Aspergillus niger |
65000 | - |
- |
Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beet arabinan + H2O | Bacillus subtilis | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Athelia rolfsii | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Bacillus subtilis F-11 | - |
L-arabinose + ? | - |
ir | |
gum arabic + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
? | |
wheat L-arabino-D-xylan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger 5195 | - |
- |
- |
Athelia rolfsii | - |
- |
- |
Bacillus subtilis | - |
F-11 | - |
Bacillus subtilis F-11 | - |
F-11 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
- |
Aspergillus niger |
- |
Athelia rolfsii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-L-arabinan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
1,5-L-arabinan + H2O | - |
Athelia rolfsii | L-arabinose + ? | - |
ir | |
1,5-L-arabinan + H2O | - |
Aspergillus niger 5195 | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Bacillus subtilis | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Athelia rolfsii | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Bacillus subtilis F-11 | L-arabinose + ? | - |
ir | |
gum arabic + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
L-arabinan + H2O | - |
Bacillus subtilis | (1,5-alpha-L-arabinose)25 + ? | - |
ir | |
L-arabinan + H2O | - |
Aspergillus niger | (1,5-alpha-L-arabinose)25 + ? | - |
ir | |
L-arabinan + H2O | - |
Bacillus subtilis F-11 | (1,5-alpha-L-arabinose)25 + ? | - |
ir | |
L-arabinan + H2O | - |
Aspergillus niger 5195 | (1,5-alpha-L-arabinose)25 + ? | - |
ir | |
additional information | - |
Aspergillus niger | ? | - |
? | |
additional information | arabinofuranosidase I does not hydrolyze L-arabinan, arabinofuranosidase II has no activity on polygalacturonic acid, carboxymethylcellulose, larch galactan, citrus pectic galactan or the synthetic substrates p-nitrophenyl derivatives of alpha- and beta-D-xylopyranosides, alpha- and beta-D-glucopyranosides, beta-D-fucopyranoside and beta-D-galactopyranoside | Bacillus subtilis | ? | - |
? | |
additional information | arabinofuranosidase I does not hydrolyze L-arabinan, arabinofuranosidase II has no activity on polygalacturonic acid, carboxymethylcellulose, larch galactan, citrus pectic galactan or the synthetic substrates p-nitrophenyl derivatives of alpha- and beta-D-xylopyranosides, alpha- and beta-D-glucopyranosides, beta-D-fucopyranoside and beta-D-galactopyranoside | Bacillus subtilis F-11 | ? | - |
? | |
additional information | - |
Aspergillus niger 5195 | ? | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | arabinofuranosidase I | Bacillus subtilis | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | arabinofuranosidase I | Bacillus subtilis F-11 | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger 5195 | p-nitrophenol + L-arabinose | - |
? | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Athelia rolfsii | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis F-11 | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger 5195 | L-arabinose + phenol | - |
ir | |
wheat L-arabino-D-xylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | enzyme is stable for 10 min at 50°C from pH 3-pH 8, at 60°C stable at pH 4 for 10 min, less stable at pH 5.5 and stable again at pH 7 | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
- |
Athelia rolfsii |
3.8 | 4 | - |
Aspergillus niger |
6.5 | - |
- |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1.5 | 10 | acid stable, even after storage at pH 2 and 5°C for 72 h, 83% enzymic activity remains | Athelia rolfsii |
7 | - |
- |
Aspergillus niger |