Application | Comment | Organism |
---|---|---|
synthesis | the G415E mutant is an excellent candidate for the industrial production of specific-length maltooligosaccharides from cyclodextrins | Pyrococcus furiosus |
Crystallization (Comment) | Organism |
---|---|
sitting drop method at 18°C. The structure studies supporting the proposition that cyclodextrin-hydrolyzing amylases from hyperthermophilic microorganisms contain all of the structural components required for substrate binding within a single monomer, distinguishing PFTA from the classical bacterial cyclodextrin-hydrolyzing enzymes of the GH13 family | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
G415E | the mutant enzyme reveals considerable differences in substrate preference relative to the wild-type enzyme. Increased substrate selectivity of the mutant enzyme for the beta-cyclodextrin substrate, whereas the mutant shows no difference in activity for the maltoheptaose substrate | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.78 | - |
beta-cyclodextrin | pH 4.5, 85°C, wild-type enzyme | Pyrococcus furiosus | |
9.07 | - |
maltoheptaose | pH 4.5, 85°C, wild-type enzyme | Pyrococcus furiosus | |
9.77 | - |
maltoheptaose | pH 4.5, 85°C, mutant enzyme G415E | Pyrococcus furiosus | |
154 | - |
beta-cyclodextrin | pH 4.5, 85°C, mutant enzyme G415E | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8TZP8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-cyclodextrin + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
maltoheptaose + H2O | - |
Pyrococcus furiosus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CD-hydrolyzing amylase | - |
Pyrococcus furiosus |
PFTA | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
assay at | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.63 | - |
maltoheptaose | pH 4.5, 85°C, mutant enzyme G415E | Pyrococcus furiosus | |
93.3 | - |
maltoheptaose | pH 4.5, 85°C, wild-type enzyme | Pyrococcus furiosus | |
199 | - |
beta-cyclodextrin | pH 4.5, 85°C, mutant enzyme G415E | Pyrococcus furiosus | |
382 | - |
beta-cyclodextrin | pH 4.5, 85°C, wild-type enzyme | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
maltoheptaose | pH 4.5, 85°C, mutant enzyme G415E | Pyrococcus furiosus | |
1.29 | - |
beta-cyclodextrin | pH 4.5, 85°C, mutant enzyme G415E | Pyrococcus furiosus | |
10.3 | - |
maltoheptaose | pH 4.5, 85°C, wild-type enzyme | Pyrococcus furiosus | |
43.5 | - |
beta-cyclodextrin | pH 4.5, 85°C, wild-type enzyme | Pyrococcus furiosus |