Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) | Evansella clarkii |
General Stability | Organism |
---|---|
more than 90% of the enzyme activity remains in the pH range of 8.0-10.0 and at 4°C in the absence of CaCl2 after pH and temperature treatments, respectively. Cda is efficiently stabilized by CaCl2. In the presence of CaCl2. Cda is stable in the pH range of 7.0-10.5 and at temperatures below 30°C. | Evansella clarkii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
gamma-cyclodextrin | - |
Evansella clarkii | |
0.067 | - |
maltooctaose | - |
Evansella clarkii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77800 | - |
calculated | Evansella clarkii |
979000 | - |
gel filtration chromatography | Evansella clarkii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Evansella clarkii | B9A1J7 | 7364 | - |
Evansella clarkii 7364 | B9A1J7 | 7364 | - |
Purification (Comment) | Organism |
---|---|
Ni-chelating chromatography | Evansella clarkii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Cda rapidly hydrolyzes only gamma-cyclodextrinase, whereas it slowly hydrolyzes alpha- and beta-cyclodextrinase, maltooctaose, amylose, amylopectin and soluble starch. The hydrolysis activities toward these substrates are less than 4% of that toward gamma-cyclodextrinase. Pullulan and glycogen are hardly hydrolyzed. Cda is a CDase that has high specificity for only gamma-cyclodextrinase | Evansella clarkii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gamma-cyclodextrin + H2O | cyclodextrinase rapidly hydrolyzes only gamma-cyclodextrinase, whereas it slowly hydrolyzes alpha- and beta-cyclodextrinase, maltooctaose, amylose, amylopectin and soluble starch. The hydrolysis activities toward these substrates are less than 4% of that toward gamma-cyclodextrinase. Pullulan and glycogen are hardly hydrolyzed. Cyclodextrinase is a CDase that has high specificity for only gamma-cyclodextrinase | Evansella clarkii | maltooctaose + ? | - |
? | |
gamma-cyclodextrin + H2O | cyclodextrinase rapidly hydrolyzes only gamma-cyclodextrinase, whereas it slowly hydrolyzes alpha- and beta-cyclodextrinase, maltooctaose, amylose, amylopectin and soluble starch. The hydrolysis activities toward these substrates are less than 4% of that toward gamma-cyclodextrinase. Pullulan and glycogen are hardly hydrolyzed. Cyclodextrinase is a CDase that has high specificity for only gamma-cyclodextrinase | Evansella clarkii 7364 | maltooctaose + ? | - |
? | |
maltooctaose + H2O | - |
Evansella clarkii | maltohexaose + maltose | small amounts of maltotriose, maltotetraose and maltopentaose are also observed | ? | |
maltooctaose + H2O | - |
Evansella clarkii 7364 | maltohexaose + maltose | small amounts of maltotriose, maltotetraose and maltopentaose are also observed | ? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 12 * 77800 | Evansella clarkii |
Synonyms | Comment | Organism |
---|---|---|
CDA | - |
Evansella clarkii |
CDase | - |
Evansella clarkii |
cyclodextrinase | - |
Evansella clarkii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Evansella clarkii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
maltooctaose | - |
Evansella clarkii | |
3100 | - |
gamma-cyclodextrin | - |
Evansella clarkii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Evansella clarkii |