Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | at 5 mM for isoenzyme ag1B* and ag1B(1Met), no activity against ag1B(2Met) | Thermotoga neapolitana |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli, three expression vectors are constructed, aglB gene linked to an upstream 90-bp 30-terminal region of the malG gene with the stop codon overlapping with the start codon of aglB, aglB with two tandem potential start codons and ag1B with only one start codon | Thermotoga neapolitana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
p-nitrophenyl-D-maltopentaoside | isoenzyme ag1B* | Thermotoga neapolitana | |
0.27 | - |
p-nitrophenyl-D-maltopentaoside | isoenzyme ag1B(1Met) | Thermotoga neapolitana | |
1.72 | - |
p-nitrophenyl-D-maltopentaoside | isoenzyme ag1B(2Met) | Thermotoga neapolitana | |
3.6 | - |
beta-cyclodextrin | isoenzyme ag1B(1Met) | Thermotoga neapolitana | |
3.9 | - |
beta-cyclodextrin | isoenzyme ag1B* | Thermotoga neapolitana | |
6.06 | - |
beta-cyclodextrin | isoenzyme ag1B(2Met) | Thermotoga neapolitana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE, aglB* | Thermotoga neapolitana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga neapolitana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by DEAE-Toyopearl chromatography and ultrafiltration | Thermotoga neapolitana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.1 | - |
isoenzyme aglB(2Met) after purification, beta-cyclodextrin as substrate | Thermotoga neapolitana |
22.4 | - |
isoenzyme aglB(1Met) after purification, beta-cyclodextrin as substrate | Thermotoga neapolitana |
22.8 | - |
isoenzyme aglB* after purification, beta-cyclodextrin as substrate | Thermotoga neapolitana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclodextrin + H2O | - |
Thermotoga neapolitana | maltooligosaccharides | maltohexaose, maltoheptaose, maltooctaose | ? | |
beta-cyclodextrin + H2O | - |
Thermotoga neapolitana | maltooligosaccharides | maltohexaose, maltoheptaose, maltooctaose | ? | |
cyclomaltodextrin + H2O | - |
Thermotoga neapolitana | linear maltodextrin | - |
? | |
gamma-cyclodextrin + H2O | preferred substrate | Thermotoga neapolitana | maltooligosaccharides | maltohexaose, maltoheptaose, maltooctaose | ? | |
p-nitrophenyl-D-maltopentaoside + H2O | - |
Thermotoga neapolitana | p-nitrophenol + maltopentaose | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 55000, aglB* | Thermotoga neapolitana |
oligomer | aglB(2Met) higher aggregated | Thermotoga neapolitana |
tetramer | 4 * 55000, aglB* | Thermotoga neapolitana |
Synonyms | Comment | Organism |
---|---|---|
AglB | - |
Thermotoga neapolitana |
CDase | - |
Thermotoga neapolitana |
cyclodextrinase | - |
Thermotoga neapolitana |
cyclomaltodextrinase | - |
Thermotoga neapolitana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermotoga neapolitana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-inactivation time of AglB(2Met) at 90°C is 5 min, for AglB* and AglB(1Met) 3 h | Thermotoga neapolitana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Thermotoga neapolitana |