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Literature summary for 3.2.1.54 extracted from
- Unusual starch degradation pathway via cyclodextrins in the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus strain 7324 (2007), J. Bacteriol., 189, 8901-8913.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | Archaeoglobus fulgidus utilizes an unusual pathway of starch degradation involving cyclodextrins as intermediates, extracellular cyclodextrins are transported into the cell and linearized via a CDase | Archaeoglobus fulgidus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | at 20 mM | Archaeoglobus fulgidus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
gamma-cyclodextrin | at 80°C, pH 4.5 | Archaeoglobus fulgidus | |
| 0.26 | - |
alpha-cyclodextrin | at 80°C, pH 4.5 | Archaeoglobus fulgidus | |
| 0.63 | - |
beta-cyclodextrin | at 80°C, pH 4.5 | Archaeoglobus fulgidus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | divalent cations required | Archaeoglobus fulgidus | |
| Co2+ | divalent cations required | Archaeoglobus fulgidus | |
| Cu2+ | inhibitory at 1 mM | Archaeoglobus fulgidus | |
| Mg2+ | divalent cations required | Archaeoglobus fulgidus | |
| Ni2+ | inhibitory at 1 mM | Archaeoglobus fulgidus | |
| Zn2+ | inhibitory at 1 mM | Archaeoglobus fulgidus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73000 | - |
SDS-PAGE | Archaeoglobus fulgidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | - |
- |
- |
| Archaeoglobus fulgidus 7324 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Q-sepharose column, DEAE-Sepharose column, Bioprep SE 1000/17 and UNO S1 column chromatography | Archaeoglobus fulgidus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.066 | - |
in the crude extract at 80°C | Archaeoglobus fulgidus |
| 36 | - |
after purification at 80°C | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | - |
Archaeoglobus fulgidus | maltooligosaccharides | maltohexaose, maltoheptaose, maltooctaose | ? | |
| alpha-cyclodextrin + H2O | - |
Archaeoglobus fulgidus 7324 | maltooligosaccharides | maltohexaose, maltoheptaose, maltooctaose | ? | |
| beta-cyclodextrin + H2O | - |
Archaeoglobus fulgidus | maltooligosaccharides | maltohexaose, maltoheptaose, maltooctaose | ? | |
| beta-cyclodextrin + H2O | - |
Archaeoglobus fulgidus 7324 | maltooligosaccharides | maltohexaose, maltoheptaose, maltooctaose | ? | |
| cyclomaltodextrin + H2O | no avtivity with activity with starch, pullulan, amylose, amylopectin, or oligosaccharides as substrates | Archaeoglobus fulgidus | linear maltodextrin | - |
? | |
| cyclomaltodextrin + H2O | no avtivity with activity with starch, pullulan, amylose, amylopectin, or oligosaccharides as substrates | Archaeoglobus fulgidus 7324 | linear maltodextrin | - |
? | |
| gamma-cyclodextrin + H2O | preferred substrate | Archaeoglobus fulgidus | maltooligosaccharides | maltohexaose, maltoheptaose, maltooctaose | ? | |
| gamma-cyclodextrin + H2O | preferred substrate | Archaeoglobus fulgidus 7324 | maltooligosaccharides | maltohexaose, maltoheptaose, maltooctaose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1*73000 | Archaeoglobus fulgidus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDase | - |
Archaeoglobus fulgidus |
| cyclodextrinase | - |
Archaeoglobus fulgidus |
| cyclomaltodextrinase | - |
Archaeoglobus fulgidus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
up to 90° | Archaeoglobus fulgidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Archaeoglobus fulgidus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | - |
Archaeoglobus fulgidus |
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