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Literature summary for 3.2.1.54 extracted from
- Chaperone-assisted refolding of Escherichia coli maltodextrin glucosidase (2007), FEBS J., 274, 6000-6010.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | chemical or molecular operones can be used in refolding of various aggregate-prone proteins of commercial and medical importance after overexpression in Escherichia coli | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| denaturation and unfolding induced with 20 mM sodium phosphate buffer, | Escherichia coli |
| denatured MalZ is enhanced five-fold | Escherichia coli |
| pH 7.0, 8 M urea and 20 mM dithiothreitol, glycerol, dimethylsulfoxide, trimethylamine-N-oxide, trehalose and proline act as chemical chaperones in the refolding of the denatured MalZ, GroEL, GroES and ATP-mediated refolding of urea | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
SDS-PAGE | Escherichia coli |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| urea | chemical chaperones inhibit denaturation induced by chaotropic agents such as urea | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Ni-chelating column HisTrap HP chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltodextrin + H2O | - |
Escherichia coli | maltose | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cyclomaltodextrinase | - |
Escherichia coli |
| maltodextrin glucosidase | MalZ | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
MalZ loses its native conformation and undergoes aggregation during incubation at 42°C., thermoprotection is achieved by proline, glycerol, dimethylsulfoxide or trimethylamine-N-oxide | Escherichia coli |
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Release 2023.1 (January 2023)
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