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Literature summary for 3.2.1.54 extracted from
- Characterisation of two novel cyclodextrinases using on-line microdialysis sampling with high-performance anion exchange chromatography (2006), Anal. Bioanal. Chem., 385, 1421-1429.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Laceyella sacchari |
| expression in Escherichia coli | Anoxybacillus flavithermus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anoxybacillus flavithermus | - |
- |
- |
| Laceyella sacchari | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant protein | Laceyella sacchari |
| of the recombinant protein | Anoxybacillus flavithermus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | - |
Laceyella sacchari | maltose + glucose | in an approximate molar ratio of 2 to 1, the intermediate products are analysed using a pulsed amperometric detector for on-line measurements and real-time sampling | ? |  |
| alpha-cyclodextrin + H2O | - |
Anoxybacillus flavithermus | maltose + glucose | in an approximate molar ratio of 2 to 1, the intermediate products are analysed using a pulsed amperometric detector for on-line measurements and real-time sampling | ? | |
| beta-cyclodextrin + H2O | - |
Laceyella sacchari | maltose + glucose | in an approximate molar ratio of 2 to 1 | ? | |
| beta-cyclodextrin + H2O | - |
Anoxybacillus flavithermus | maltose + glucose | in an approximate molar ratio of 2 to 1 | ? | |
| cyclomaltodextrin + H2O | - |
Laceyella sacchari | linear maltodextrin | - |
? | |
| cyclomaltodextrin + H2O | - |
Anoxybacillus flavithermus | linear maltodextrin | - |
? | |
| gamma-cyclodextrin + H2O | - |
Laceyella sacchari | maltose + glucose | in an approximate molar ratio of 2 to 1 | ? | |
| gamma-cyclodextrin + H2O | - |
Anoxybacillus flavithermus | maltose + glucose | in an approximate molar ratio of 2 to 1 | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Anoxybacillus flavithermus |
| monomer | - |
Laceyella sacchari |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AfCda13 | - |
Anoxybacillus flavithermus |
| CDase | - |
Laceyella sacchari |
| CDase | - |
Anoxybacillus flavithermus |
| cyclomaltodextrinase | - |
Laceyella sacchari |
| cyclomaltodextrinase | - |
Anoxybacillus flavithermus |
| LsCda13 | - |
Laceyella sacchari |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Laceyella sacchari |
| 55 | - |
- |
Anoxybacillus flavithermus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Laceyella sacchari |
| 7 | - |
- |
Anoxybacillus flavithermus |
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Release 2023.1 (January 2023)
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