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Literature summary for 3.2.1.54 extracted from
- Two novel cyclodextrin-degrading enzymes isolated from thermophilic bacteria have similar domain structures but differ in oligomeric state and activity profile (2005), J. Biosci. Bioeng., 100, 380-390.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Laceyella sacchari |
| expression in Escherichia coli | Anoxybacillus flavithermus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
alpha-cyclodextrin | pH 6.5, 55°C | Anoxybacillus flavithermus |  |
| 0.32 | - |
beta-cyclodextrin | pH 6.5, 55°C | Anoxybacillus flavithermus | |
| 5 | - |
soluble starch | pH 6, 55°C | Laceyella sacchari | |
| 7.9 | - |
alpha-cyclodextrin | pH 6, 55°C | Laceyella sacchari | |
| 11 | - |
soluble starch | pH 6.5, 55°C | Anoxybacillus flavithermus | |
| 12 | - |
beta-cyclodextrin | pH 6, 55°C | Laceyella sacchari | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 71000 | - |
2 * 71000, at pH 6.5, the enzyme exiosts mainly as dimer, SDS-PAGE | Laceyella sacchari |
| 71000 | - |
2 * 71000, at pH 6.5, the enzyme exiosts mainly as dimer, SDS-PAGE | Anoxybacillus flavithermus |
| 148000 | - |
gel filtration | Laceyella sacchari |
| 148000 | - |
gel filtration | Anoxybacillus flavithermus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anoxybacillus flavithermus | Q5BLZ6 | - |
- |
| Laceyella sacchari | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Laceyella sacchari |
- |
Anoxybacillus flavithermus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | - |
Laceyella sacchari | D-glucose + maltose | - |
? | |
| alpha-cyclodextrin + H2O | preferred substrate. As compared with the dimer protein, the monomeric enzyme retains only 23% of the specific activity | Anoxybacillus flavithermus | D-glucose + maltose | + maltotriose + maltotetraose + maltoheptaose at lower amounts | ? | |
| amylose + H2O | - |
Laceyella sacchari | ? | - |
? | |
| amylose + H2O | - |
Anoxybacillus flavithermus | ? | - |
? | |
| beta-cyclodextrin + H2O | - |
Anoxybacillus flavithermus | D-glucose + maltose | + maltotriose + maltotetraose + maltoheptaose at lower amounts | ? | |
| beta-cyclodextrin + H2O | preferred substrate | Laceyella sacchari | D-glucose + maltose | - |
? | |
| pullulan + H2O | - |
Laceyella sacchari | ? | - |
? | |
| pullulan + H2O | - |
Anoxybacillus flavithermus | ? | - |
? | |
| soluble starch + H2O | - |
Laceyella sacchari | ? | - |
? | |
| soluble starch + H2O | - |
Anoxybacillus flavithermus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 71000, at pH 6.5, the enzyme exiosts mainly as dimer, SDS-PAGE | Laceyella sacchari |
| dimer | 2 * 71000, at pH 6.5, the enzyme exiosts mainly as dimer, SDS-PAGE | Anoxybacillus flavithermus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AfCda13 | - |
Anoxybacillus flavithermus |
| LsCda13 | - |
Laceyella sacchari |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 57 | - |
- |
Laceyella sacchari |
| 57.5 | - |
- |
Anoxybacillus flavithermus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 70 | 45°C: about 50% of maximal activity, 70°C: about 70% of maximal activitry | Anoxybacillus flavithermus |
| 52 | 60 | 52°C: about 50% of maximal activity, 60°C: about 30% of maximal activitry | Laceyella sacchari |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 57 | - |
complete loss of activity after 20 min without calcium, completely stable after 120 min in presence of 1 mM calcium | Laceyella sacchari |
| 60 | - |
half-life: 20 min. Thermal inactivation is not significantly affected by addition of calcium | Anoxybacillus flavithermus |
| 60 | - |
half-life: 4 min, in absence of calcium. 50% loss of activity after 120 min in presence of 1 mM calcium | Laceyella sacchari |
| 67 | - |
complete loss of activity despite presence of calcium | Laceyella sacchari |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.2 | - |
beta-cyclodextrin | pH 6, 55°C | Laceyella sacchari | |
| 3.6 | - |
pullulan | pH 6.5, 55°C | Anoxybacillus flavithermus | |
| 13.4 | - |
amylose | pH 6.5, 55°C | Anoxybacillus flavithermus | |
| 16.9 | - |
soluble starch | pH 6.5, 55°C | Anoxybacillus flavithermus | |
| 23 | - |
amylose | pH 6, 55°C | Laceyella sacchari | |
| 36.8 | - |
soluble starch | pH 6, 55°C | Laceyella sacchari | |
| 42.6 | - |
pullulan | pH 6, 55°C | Laceyella sacchari | |
| 46.1 | - |
alpha-cyclodextrin | pH 6, 55°C | Laceyella sacchari | |
| 73.7 | - |
beta-cyclodextrin | pH 6, 55°C | Laceyella sacchari | |
| 315 | - |
beta-cyclodextrin | pH 6.5, 55°C | Anoxybacillus flavithermus | |
| 545 | - |
alpha-cyclodextrin | pH 6.5, 55°C | Anoxybacillus flavithermus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Laceyella sacchari |
| 6.5 | - |
- |
Anoxybacillus flavithermus |
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