Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression as native and selenomethionine derivatives in Eschrichia coli BL21(DE3) and B834 (DE3), respectively | Flavobacterium sp. |
Crystallization (Comment) | Organism |
---|---|
6 mg/ml purified recombinant enzyme as native and selenomethionine derivatives in 50 mM HEPES, pH 7.5, 3.8 M NaCl, and 3 mM DTT with the SeM-enzyme derivative, equal volumes of protein and reservoir solution, hanging drop vapour diffusion method, 2 days, 15% glycerol for cryoprotection, multiwavelength anomalous X-ray diffraction 3D-structure determination and analysis at 2.1 A resolution | Flavobacterium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | determination of the binding site | Flavobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | Q8KKG0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Flavobacterium sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cyclomaltodextrin + H2O = linear maltodextrin | active site structure | Flavobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclomaltodextrin + H2O | - |
Flavobacterium sp. | alpha-D-glucose + maltose | - |
? | |
beta-cyclomaltodextrin + H2O | - |
Flavobacterium sp. | alpha-D-glucose + maltose | - |
? | |
gamma-cyclomaltodextrin + H2O | - |
Flavobacterium sp. | alpha-D-glucose + maltose | - |
? | |
pullulan + H2O | - |
Flavobacterium sp. | ? | - |
? | |
starch + H2O | - |
Flavobacterium sp. | alpha-D-glucose + maltose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CD-degrading enzyme | - |
Flavobacterium sp. |
CDase | - |
Flavobacterium sp. |
cyclodextrinase | - |
Flavobacterium sp. |
More | enzyme belongs to the glycosylhydrolase family 13 | Flavobacterium sp. |