Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 5 mM EDTA, activity increases more than 2fold, relative activity 237.2% | Bacillus sp. (in: Bacteria) | |
EGTA | increases activity about 2.3fold, relative activity 236.3% | Bacillus sp. (in: Bacteria) |
Cloned (Comment) | Organism |
---|---|
CDase I-5 gene, pBR322, expression in Escherichia coli MC1061 | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Bacillus sp. (in: Bacteria) | |
Cr3+ | relative activity 58.4% | Bacillus sp. (in: Bacteria) | |
Cu2+ | - |
Bacillus sp. (in: Bacteria) | |
Fe3+ | relative activity 37.7% | Bacillus sp. (in: Bacteria) | |
Li+ | relative activity 75.5% | Bacillus sp. (in: Bacteria) | |
Mg2+ | relative activity 43% | Bacillus sp. (in: Bacteria) | |
Mn2+ | relative activity 64.2% | Bacillus sp. (in: Bacteria) | |
Ni2+ | relative activity 72.2% | Bacillus sp. (in: Bacteria) | |
Zn2+ | relative activity 5.6% | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | relative activity 101.5% | Bacillus sp. (in: Bacteria) | |
Ca2+ | activated and stabilized by Ca2+, relative activity 128.7% | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
MW deduced from the nucleotide sequence 64884 | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
I-5 | - |
Bacillus sp. (in: Bacteria) I-5 | - |
I-5 | - |
Purification (Comment) | Organism |
---|---|
gene encoding enzyme cloned in Escherichia coli, expressed and purified from Escherichia coli | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
148 | - |
crude enzyme, purification 1fold | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acarbose + H2O | pseudotetrasaccharide inhibitor of glucosidases and alpha-amylases, binding the active sites of the enzyme | Bacillus sp. (in: Bacteria) | ? | - |
? | |
acarbose + H2O | pseudotetrasaccharide inhibitor of glucosidases and alpha-amylases, binding the active sites of the enzyme | Bacillus sp. (in: Bacteria) I-5 | ? | - |
? | |
cyclomaltodextrin + H2O | - |
Bacillus sp. (in: Bacteria) | maltodextrin | - |
? | |
cyclomaltodextrin + H2O | - |
Bacillus sp. (in: Bacteria) I-5 | maltodextrin | - |
? | |
pullulan + H2O | - |
Bacillus sp. (in: Bacteria) | glucose + panose + isopanose + large saccharides containing alpha 1,6-glucosidic bonds | - |
? | |
pullulan + H2O | - |
Bacillus sp. (in: Bacteria) I-5 | glucose + panose + isopanose + large saccharides containing alpha 1,6-glucosidic bonds | - |
? | |
starch + H2O | - |
Bacillus sp. (in: Bacteria) | glucose + maltose | - |
? | |
starch + H2O | - |
Bacillus sp. (in: Bacteria) I-5 | glucose + maltose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | in the presence of Ca2+ | Bacillus sp. (in: Bacteria) |
50 | - |
pullulan, optimum temperature variable depending on the substrate | Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | - |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
thermal activity more than 80% in presence of Ca2+ | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | pullulan as substrate | Bacillus sp. (in: Bacteria) |
6 | - |
soluble starch, the optimum pH depending on the substrate | Bacillus sp. (in: Bacteria) |
7 | - |
beta-cyclodextrin as substrate | Bacillus sp. (in: Bacteria) |