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Literature summary for 3.2.1.54 extracted from
- Metabolism of cyclodextrins by Klebsiella oxytoca M5a1: purification and characterisation of a cytoplasmically located cyclodextrinase (1996), Arch. Microbiol., 165, 206-212.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cymH, pCYMH, expression in Escherichia coli JM109 | Klebsiella oxytoca |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
gamma-cyclodextrin | - |
Klebsiella oxytoca |  |
| 3.9 | - |
beta-cyclodextrin | - |
Klebsiella oxytoca | |
| 50 | - |
alpha-cyclodextrin | - |
Klebsiella oxytoca | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Klebsiella oxytoca | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
gel filtration | Klebsiella oxytoca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella oxytoca | - |
M5a1 | - |
| Klebsiella oxytoca M5a1 | - |
M5a1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Klebsiella oxytoca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | - |
Klebsiella oxytoca | maltose + maltotriose | - |
? | |
| alpha-cyclodextrin + H2O | - |
Klebsiella oxytoca M5a1 | maltose + maltotriose | - |
? | |
| beta-cyclodextrin + H2O | - |
Klebsiella oxytoca | maltose + maltotriose | - |
? | |
| beta-cyclodextrin + H2O | - |
Klebsiella oxytoca M5a1 | maltose + maltotriose | - |
? | |
| cyclomaltodextrin + H2O | - |
Klebsiella oxytoca | maltodextrin | - |
? | |
| cyclomaltodextrin + H2O | - |
Klebsiella oxytoca M5a1 | maltodextrin | - |
? | |
| gamma-cyclodextrin + H2O | - |
Klebsiella oxytoca | maltose + maltotriose | - |
? | |
| gamma-cyclodextrin + H2O | - |
Klebsiella oxytoca M5a1 | maltose + maltotriose | - |
? | |
| maltodextrins + H2O | - |
Klebsiella oxytoca | maltose + glucose | - |
? | |
| maltodextrins + H2O | - |
Klebsiella oxytoca M5a1 | maltose + glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
- |
Klebsiella oxytoca |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 34 | - |
Klebsiella oxytoca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
irreversibel inactivated at 40°C | Klebsiella oxytoca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
crude enzyme | Klebsiella oxytoca |
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