Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-deoxy-D-glucose | 0.01 M, relative activity 140% | Lysinibacillus sphaericus | |
glucosamine | 0.01 M, relative activity 133% | Lysinibacillus sphaericus | |
glucose | 0.01 M, relative activity 143% | Lysinibacillus sphaericus | |
glucose 6-phosphate | 0.005 M, relative activity 111% | Lysinibacillus sphaericus | |
maltose | 0.01 M, relative activity 140% | Lysinibacillus sphaericus | |
miglitol | 1 mg/ml, relative activity 107% | Lysinibacillus sphaericus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acarbose | - |
Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
beta-cyclodextrin | - |
Lysinibacillus sphaericus | |
4 | - |
alpha-cyclodextrin | - |
Lysinibacillus sphaericus | |
4.2 | - |
gamma-cyclodextrin | - |
Lysinibacillus sphaericus | |
5.8 | - |
maltoheptaose | - |
Lysinibacillus sphaericus | |
6.1 | - |
maltohexaose | - |
Lysinibacillus sphaericus | |
7.5 | - |
maltotetraose | - |
Lysinibacillus sphaericus | |
14 | - |
maltotriose | - |
Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
91200 | 95000 | gel filtration | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
ATCC 7055 | - |
Purification (Comment) | Organism |
---|---|
- |
Lysinibacillus sphaericus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
366.3 | - |
homogenous by SDS-PAGE | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclomaltodextrin + H2O | - |
Lysinibacillus sphaericus | alpha-D-glucose + maltose | - |
? | |
beta-cyclodextrin + H2O | - |
Lysinibacillus sphaericus | maltose + glucose | - |
? | |
beta-cyclodextrin + H2O | - |
Lysinibacillus sphaericus | glucose + maltose + maltotriose + maltotetraose | - |
? | |
branched cyclodextrins + H2O | - |
Lysinibacillus sphaericus | glucose + maltose | - |
? | |
cyclomaltodextrin + H2O | - |
Lysinibacillus sphaericus | maltodextrin | - |
? | |
gamma-cyclodextrin + H2O | - |
Lysinibacillus sphaericus | glucose + maltose + maltotriose + maltotetraose + maltopentaose + maltohexaose + maltoheptaose + maltooctaose | - |
? | |
glucosyl-beta-cyclodextrin + H2O | - |
Lysinibacillus sphaericus | 6,4-O-alpha-D-glucosylmaltoheptaose | - |
? | |
maltoheptaose + H2O | - |
Lysinibacillus sphaericus | D-glucose + maltose + ? | - |
? | |
maltopentaose + H2O | - |
Lysinibacillus sphaericus | maltose + maltotriose | - |
? | |
maltosyl-alpha-cyclodextrin + H2O | - |
Lysinibacillus sphaericus | 6,3-O-alpha-D-maltosylmaltohexaose | - |
? | |
maltosyl-beta-cyclodextrin | - |
Lysinibacillus sphaericus | 6,4-O-alpha-D-maltosylmaltoheptaose | - |
? | |
pullulan + H2O | - |
Lysinibacillus sphaericus | glucose + panose + isopanose + large saccharides containing alpha 1,6-glucosidic bonds | - |
? | |
starch + H2O | - |
Lysinibacillus sphaericus | glucose + maltose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Lysinibacillus sphaericus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
100% stable for 30 min up to 40°C | Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | 97% activity at pH 7 | Lysinibacillus sphaericus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | 100% stable | Lysinibacillus sphaericus |