Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | enzyme activity completely inhibited in the presence of 10 mM CuSO4 | Bacillus sp. (in: Bacteria) | |
Fe2+ | enzyme activity completely inhibited in the presence of 10 mM FeSO4 | Bacillus sp. (in: Bacteria) | |
N-acetylgalactosamine | relative activity in presence of 10 mM 50%, in presence of 50 mM 12% | Bacillus sp. (in: Bacteria) | |
Urea | relative activity in presence of 2 M 32% | Bacillus sp. (in: Bacteria) | |
Zn2+ | enzyme activity completely inhibited in the presence of 10 mM ZnSO4 | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
4-nitrophenyl beta-acetylgalactosaminide | - |
Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | relative activity in presence of MgSO4 10 mM 104% | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
SDS-PAGE, enzyme denatured with SDS and 2-mercaptoethanol | Bacillus sp. (in: Bacteria) |
54000 | - |
native enzyme, gel filtration | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-N-acetylgalactosaminylated oligosaccharides + H2O | Bacillus sp. (in: Bacteria) | - |
? | - |
? | |
beta-N-acetylgalactosaminylated oligosaccharides + H2O | Bacillus sp. (in: Bacteria) AT173-1 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
AT173-1 | - |
Bacillus sp. (in: Bacteria) AT173-1 | - |
AT173-1 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | AT 173-1, culture broth centrifuged to remove cells | Bacillus sp. (in: Bacteria) | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.4 | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-acetylgalactosaminide + H2O | - |
Bacillus sp. (in: Bacteria) | 4-nitrophenol + beta-acetylgalactosamine | - |
? | |
4-nitrophenyl beta-acetylgalactosaminide + H2O | - |
Bacillus sp. (in: Bacteria) AT173-1 | 4-nitrophenol + beta-acetylgalactosamine | - |
? | |
beta-N-acetylgalactosaminylated oligosaccharides + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
beta-N-acetylgalactosaminylated oligosaccharides + H2O | - |
Bacillus sp. (in: Bacteria) AT173-1 | ? | - |
? | |
N-acetyl-beta-D-galactosaminides + H2O | substrate specificity towards beta-GalNAc residues is markedly higher than those of any other beta-GalNacase | Bacillus sp. (in: Bacteria) | N-acetyl-D-galactosamine + an alcohol | - |
? | |
N-acetyl-beta-D-galactosaminides + H2O | substrate specificity towards beta-GalNAc residues is markedly higher than those of any other beta-GalNacase | Bacillus sp. (in: Bacteria) AT173-1 | N-acetyl-D-galactosamine + an alcohol | - |
? | |
p-nitrophenyl beta-N-acetylgalactosaminide + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
p-nitrophenyl beta-N-acetylgalactosaminide + H2O | - |
Bacillus sp. (in: Bacteria) AT173-1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bacillus beta-GlcNAcase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 45 | enzyme gradually inactivated in solution at 4°C, reactivated by incubation with DTT | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
4-methylumbelliferyl beta-N-acetylgalactosaminide | Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | enzyme retains more than 80% activity | Bacillus sp. (in: Bacteria) |