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Literature summary for 3.2.1.52 extracted from

  • Wang, M.; Zheng, F.; Wang, T.; Lyu, Y.M.; Alteen, M.G.; Cai, Z.P.; Cui, Z.L.; Liu, L.; Voglmeir, J.
    Characterization of Stackebrandtia nassauensis GH 20 beta-hexosaminidase, a versatile biocatalyst for chitobiose degradation (2019), Int. J. Mol. Sci., 20, 1243 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.52

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Stackebrandtia nassauensis

Protein Variants

Protein Variants Comment Organism
D306A the mutant enzyme is inactive with 4-nitrophenyl N-acetyl-beta-D-glucosaminide and chitobiose Stackebrandtia nassauensis
D306E the mutant enzyme shows 4.2% activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide compared to the wild type and is inactive with chitobiose Stackebrandtia nassauensis
D306E/E307D the mutant enzyme shows 2.7% activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide compared to the wild type and is inactive with chitobiose Stackebrandtia nassauensis
D306N the mutant enzyme is inactive with 4-nitrophenyl N-acetyl-beta-D-glucosaminide and chitobiose Stackebrandtia nassauensis
E307A the mutant enzyme shows 2.0% activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide compared to the wild type and is inactive with chitobiose Stackebrandtia nassauensis
E307D the mutant enzyme shows 8.6% activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide compared to the wild type and is inactive with chitobiose Stackebrandtia nassauensis
E307Q the mutant enzyme shows 1.5% activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide compared to the wild type and is inactive with chitobiose Stackebrandtia nassauensis

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by EDTA, 2-mercaptoethanol, imidazole, iodoacetamide, or Triton X-100 Stackebrandtia nassauensis
PUGNAc i.e. O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate Stackebrandtia nassauensis
SDS 58% inhibition at 1% (w/v) Stackebrandtia nassauensis
Urea 44% inhibition at 1 mM Stackebrandtia nassauensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.47
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide at pH 6.0 and 37°C Stackebrandtia nassauensis
3.29
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide at pH 6.0 and 37°C Stackebrandtia nassauensis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the addition of divalent metal ions has no effect on the enzymatic activity Stackebrandtia nassauensis

Organism

Organism UniProt Comment Textmining
Stackebrandtia nassauensis D3PZ76
-
-
Stackebrandtia nassauensis DSM 44728 D3PZ76
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA Sefinose resin column chromatography Stackebrandtia nassauensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Stackebrandtia nassauensis 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Stackebrandtia nassauensis DSM 44728 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Stackebrandtia nassauensis 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Stackebrandtia nassauensis DSM 44728 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
chitobiose + H2O
-
 Stackebrandtia nassauensis 2 D-glucosamine
-
 ?
chitobiose + H2O
-
 Stackebrandtia nassauensis DSM 44728 2 D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
? x * 57800 + x * 29000, MALDI-TOF mass spectrometry Stackebrandtia nassauensis
? x * 58000, calculated from amino acid sequence Stackebrandtia nassauensis
? x * 70-100000, SDS-PAGE Stackebrandtia nassauensis

Synonyms

Synonyms Comment Organism
Hex
-
 Stackebrandtia nassauensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Stackebrandtia nassauensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 about 50% activity at 40°C, about 90% activity at 50°C, 100% at 60°C, about 90% activity at 70°C, less than 20% activity at 80°C Stackebrandtia nassauensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 60 the enzyme is stable for at least 24 h at 37°C and 50°C. Preincubation of the enzyme at 60°C leads to its complete inactivation within 1 h Stackebrandtia nassauensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.71
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide at pH 6.0 and 37°C Stackebrandtia nassauensis
0.92
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide at pH 6.0 and 37°C Stackebrandtia nassauensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Stackebrandtia nassauensis

pH Range

pH Minimum pH Maximum Comment Organism
4 8 more than 50% activity between pH 4.0 and 8.0 Stackebrandtia nassauensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.22
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide at pH 6.0 and 37°C Stackebrandtia nassauensis
0.37
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide at pH 6.0 and 37°C Stackebrandtia nassauensis