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Literature summary for 3.2.1.52 extracted from

  • Wang, M.; Zhang, X.Y.; Guo, R.R.; Cai, Z.P.; Hu, X.C.; Chen, H.; Wei, S.; Voglmeir, J.; Liu, L.
    Cloning, purification and biochemical characterization of two beta-N-acetylhexosaminidases from the mucin-degrading gut bacterium Akkermansia muciniphila (2018), Carbohydr. Res., 457, 1-7 .
    View publication on PubMed
Search for more literature for 3.2.1.52

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Akkermansia muciniphila

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ about 70% inhibition of isoform Am2301 at 2 mM, about 30% inhibition of isoform Am2446 at 2 mM Akkermansia muciniphila
EDTA about 40% inhibition of isoform Am2301 at 2 mM, isoform Am2446 is not inhibited by EDTA Akkermansia muciniphila
Fe3+ about 60% inhibition of isoform Am2446 at 2 mM, about 15% inhibition of isoform Am2301 at 2 mM Akkermansia muciniphila
additional information the addition of H2O2, 2-mercaptoethanol, urea, Triton X-100, N-ethylmaleimide or 2-iodoacetamide has no or little effect on the activities of the enzymes Akkermansia muciniphila
PUGNAc i.e. O-(2-acetamido-2-deoxy-D-glucopyranosylidenamino) N-phenylcarbamate, complete inhibition at 0.001 mM Akkermansia muciniphila
SDS almost complete inhibition at 1.0-0.1% (v/v) SDS Akkermansia muciniphila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide isoform Am2301, at pH 5.0 and 37°C Akkermansia muciniphila
0.38
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide isoform Am2446, at pH 6.5 and 37°C Akkermansia muciniphila
0.52
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide isoform Am2301, at pH 5.0 and 37°C Akkermansia muciniphila
1.36
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide isoform Am2446, at pH 6.5 and 37°C Akkermansia muciniphila

Organism

Organism UniProt Comment Textmining
Akkermansia muciniphila
-
-
-
Akkermansia muciniphila DSM 22959
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Akkermansia muciniphila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
44
-
 isoform Am2301, with 4-nitrophenyl N-acetyl-beta-D-glucosaminide as substrate, at pH 5.0 and 37°C Akkermansia muciniphila
170
-
 isoform Am2446, with 4-nitrophenyl N-acetyl-beta-D-glucosaminide as substrate, at pH 6.5 and 37°C Akkermansia muciniphila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Akkermansia muciniphila 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Akkermansia muciniphila DSM 22959 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Akkermansia muciniphila 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Akkermansia muciniphila DSM 22959 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
Gly-L-Glu-N-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-Asn-L-Arg + H2O efficient substrate for isoforms Am2301 and Am2446 Akkermansia muciniphila N-acetyl-beta-D-glucosamine + ?
-
 ?
Gly-L-Glu-N-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-Asn-L-Arg + H2O efficient substrate for isoforms Am2301 and Am2446 Akkermansia muciniphila DSM 22959 N-acetyl-beta-D-glucosamine + ?
-
 ?
Gly-L-Glu-N-[beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-Asn-L-Arg + H2O isoform Am2301 shows moderate hydrolytic activity Akkermansia muciniphila N-acetyl-beta-D-glucosamine + ?
-
 ?
additional information no activity with 4-nitrophenyl beta-D-glucose, 4-nitrophenyl beta-D-galactose, 4-nitrophenyl alpha-D-glucose, 4-nitrophenyl alpha-D-galactose, 4-nitrophenyl N-acetyl-alpha-D-glucosaminide, and 4-nitrophenyl alpha-D-fucose Akkermansia muciniphila ?
-
 ?
additional information the enzymes catalyze the hydrolysis of beta1,4-linked N-acetylgalactosamine and beta1,6-linked N-acetylglucosamine from glycan standards, as well as beta1,2-linked N-acetylglucosamine units from the non-reducing end of N-glycans Akkermansia muciniphila ?
-
 ?
additional information no activity with 4-nitrophenyl beta-D-glucose, 4-nitrophenyl beta-D-galactose, 4-nitrophenyl alpha-D-glucose, 4-nitrophenyl alpha-D-galactose, 4-nitrophenyl N-acetyl-alpha-D-glucosaminide, and 4-nitrophenyl alpha-D-fucose Akkermansia muciniphila DSM 22959 ?
-
 ?
additional information the enzymes catalyze the hydrolysis of beta1,4-linked N-acetylgalactosamine and beta1,6-linked N-acetylglucosamine from glycan standards, as well as beta1,2-linked N-acetylglucosamine units from the non-reducing end of N-glycans Akkermansia muciniphila DSM 22959 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, isoform Am2301, SDS-PAGE Akkermansia muciniphila
? x * 78000, isoform Am2446, SDS-PAGE Akkermansia muciniphila

Synonyms

Synonyms Comment Organism
Am2301
-
 Akkermansia muciniphila
Am2446
-
 Akkermansia muciniphila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Akkermansia muciniphila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 42 isoform Am2301 retains more than 90% activity after 96 h of incubation at 37°C, and for 12 h incubation at 42°C. The activity of isoform Am2446 decreases already 90% within 2 h of incubation at 37°C Akkermansia muciniphila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 isoform Am2301 Akkermansia muciniphila
6.5
-
 isoform Am2446 Akkermansia muciniphila

pH Range

pH Minimum pH Maximum Comment Organism
4.5 5.5 the activity of isoform Am2301 sharply drops below pH 4.5 and above pH 5.5 Akkermansia muciniphila

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0005
-
 isoform Am2301, at 5.0 pH and 37°C Akkermansia muciniphila PUGNAc
0.0005
-
 isoform Am2446, at 6.5 pH and 37°C Akkermansia muciniphila PUGNAc