Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain X33 | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | Bacillus subtilis | - |
N-acetyl-beta-D-glucosamine | - |
? | |
chitin + H2O | Bacillus subtilis 168 | - |
N-acetyl-beta-D-glucosamine | - |
? | |
chitosan + H2O | Bacillus subtilis | - |
N-acetyl-beta-D-glucosamine | - |
? | |
chitosan + H2O | Bacillus subtilis 168 | - |
N-acetyl-beta-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P40406 | - |
- |
Bacillus subtilis 168 | P40406 | - |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Bacillus subtilis | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Bacillus subtilis 168 | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
chitin + H2O | - |
Bacillus subtilis | N-acetyl-beta-D-glucosamine | - |
? | |
chitin + H2O | - |
Bacillus subtilis 168 | N-acetyl-beta-D-glucosamine | - |
? | |
chitosan + H2O | - |
Bacillus subtilis | N-acetyl-beta-D-glucosamine | - |
? | |
chitosan + H2O | - |
Bacillus subtilis 168 | N-acetyl-beta-D-glucosamine | - |
? | |
colloidal chitin + H2O | - |
Bacillus subtilis | N-acetyl-beta-D-glucosamine + ? | - |
? | |
colloidal chitin + H2O | - |
Bacillus subtilis 168 | N-acetyl-beta-D-glucosamine + ? | - |
? | |
N,N'-diacetylchitobiose + H2O | - |
Bacillus subtilis | N-acetyl-beta-D-glucosamine | - |
? | |
N,N'-diacetylchitobiose + H2O | - |
Bacillus subtilis 168 | N-acetyl-beta-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
beta-N-acetylglucosaminidase | - |
Bacillus subtilis |
NAGase | - |
Bacillus subtilis |
NagZ | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 65 | the enzyme maintains over 60% relative activity from 45 to 65°C | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | the enzyme keeps over 80% activity after pre-incubation at 55°C for 1 h and 60% residual activity after 10 min pre-incubation at 60°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | the enzyme remains over 60% relative activity from pH 5.5-7.0 | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10 | the enzyme keeps over 80% activity after pre-incubation at 4°C for 12 h from pH 4.5-10.0 | Bacillus subtilis |