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Literature summary for 3.2.1.52 extracted from

  • Alteen, M.G.; Oehler, V.; Nemcovicova, I.; Wilson, I.B.; Vocadlo, D.J.; Gloster, T.M.
    Mechanism of human nucleocytoplasmic hexosaminidase D (2016), Biochemistry, 55, 2735-2747 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.52

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D148A the mutant shows 868fold decreased catalytic efficiency with 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide compared to the wild type enzyme Homo sapiens
D148N the mutant shows 4190fold decreased catalytic efficiency with 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide compared to the wild type enzyme Homo sapiens
E149A the mutant shows 1.6fold decreased catalytic efficiency with 4-nitrophenyl N-acetyl-beta-D-galactosaminide and 0.85fold increased catalytic efficiency with 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide compared to the wild type enzyme Homo sapiens
E149Q the mutant shows 2.2fold decreased catalytic efficiency with 4-nitrophenyl N-acetyl-beta-D-galactosaminide compared to the wild type enzyme Homo sapiens
H92A the mutant shows 69fold decreased catalytic efficiency with 4-nitrophenyl N-acetyl-beta-D-galactosaminide compared to the wild type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Gal-NAG-thiazoline
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0525
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149A, at pH 7.4 and 37°C Homo sapiens
0.0721
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.102
-
 4-nitrophenyl 2-deoxy-2-difluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.103
-
 4-nitrophenyl 2-deoxy-2-trifluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.172
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.175
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149Q, at pH 7.4 and 37°C Homo sapiens
0.196
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme H92A, at pH 7.4 and 37°C Homo sapiens
0.213
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149A, at pH 7.4 and 37°C Homo sapiens
0.533
-
 4-nitrophenyl 2-deoxy-2-fluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.566
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme D148A, at pH 7.4 and 37°C Homo sapiens
0.607
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.852
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
1.73
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme D148N, at pH 7.4 and 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleoplasm
-
 Homo sapiens 5654
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap nickel column chromatography and S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Homo sapiens 3-fluoro-4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Homo sapiens 3-fluoro-4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
4-nitrophenyl 2-deoxy-2-difluoroacetamido-beta-D-galactopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + 2-deoxy-2-difluoroacetamido-beta-D-galactopyranose
-
 ?
4-nitrophenyl 2-deoxy-2-fluoroacetamido-beta-D-galactopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + 2-deoxy-2-fluoroacetamido-beta-D-galactopyranose
-
 ?
4-nitrophenyl 2-deoxy-2-trifluoroacetamido-beta-D-galactopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + 2-deoxy-2-trifluoroacetamido-beta-D-galactopyranose
-
 ?
4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Homo sapiens 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Homo sapiens 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?

Synonyms

Synonyms Comment Organism
HexD
-
 Homo sapiens
HEXDC
-
 Homo sapiens
hexosaminidase D
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0048
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme D148N, at pH 7.4 and 37°C Homo sapiens
0.0077
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme D148A, at pH 7.4 and 37°C Homo sapiens
0.042
-
 4-nitrophenyl 2-deoxy-2-trifluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.0515
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme H92A, at pH 7.4 and 37°C Homo sapiens
0.27
-
 4-nitrophenyl 2-deoxy-2-difluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.51
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.6016
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149A, at pH 7.4 and 37°C Homo sapiens
1.32
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
1.4883
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149Q, at pH 7.4 and 37°C Homo sapiens
1.54
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
2.03
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
2.6
-
 4-nitrophenyl 2-deoxy-2-fluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
2.9333
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149A, at pH 7.4 and 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00042
-
 Gal-NAG-thiazoline wild type enzyme, at pH 7.4 and 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0028
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme D148N, at pH 7.4 and 37°C Homo sapiens
0.0136
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme D148A, at pH 7.4 and 37°C Homo sapiens
0.26
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme H92A, at pH 7.4 and 37°C Homo sapiens
0.41
-
 4-nitrophenyl 2-deoxy-2-trifluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.835
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
1.817
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
2.65
-
 4-nitrophenyl 2-deoxy-2-difluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
4.98
-
 4-nitrophenyl 2-deoxy-2-fluoroacetamido-beta-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
8.47
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149Q, at pH 7.4 and 37°C Homo sapiens
11.52
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149A, at pH 7.4 and 37°C Homo sapiens
11.783
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens
13.75
-
 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide mutant enzyme E149A, at pH 7.4 and 37°C Homo sapiens
18.167
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide wild type enzyme, at pH 7.4 and 37°C Homo sapiens