Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain KM71H | Aspergillus versicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2,3-tris-[1-(2-acetamido-2-deoxy-beta-D-galactopyranosyl-(1->4)-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-1H-1,2,3-triazol-4-yl)-2-ethyloxy]benzene | - |
Aspergillus versicolor | |
beta-D-GalNAc-(1->4)beta-D-GlcNAc | - |
Aspergillus versicolor | |
lactose | - |
Aspergillus versicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | recombinant enzyme, at pH 5.0 and 25°C | Aspergillus versicolor | |
0.22 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | wild type enzyme, at pH 5.0 and 25°C | Aspergillus versicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
MALDI TOF mass spectrometry | Aspergillus versicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus versicolor | A0A1L9P8I4 | - |
- |
Aspergillus versicolor CCF 2491 | A0A1L9P8I4 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Aspergillus versicolor |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography | Aspergillus versicolor |
Storage Stability | Organism |
---|---|
4°C, purified and concentrated enzyme in McIlvaine buffer pH 5.0 conserved with 0.03% (w/v) NaN3, ca 6 months, less than 20% loss of activity | Aspergillus versicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Aspergillus versicolor | 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose | - |
? | |
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Aspergillus versicolor CCF 2491 | 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Aspergillus versicolor | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Aspergillus versicolor CCF 2491 | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56872, catalytic domain, calculated from amino acid sequence | Aspergillus versicolor |
heterodimer | 1 * 78000 + 1 * 64000, SDS-PAGE | Aspergillus versicolor |
Synonyms | Comment | Organism |
---|---|---|
Hex | - |
Aspergillus versicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Aspergillus versicolor |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | the recombinant enzyme shows more than 80% activity between 40 and 60°C. The wild type enzyme shows about 65% activity at 45°C, 90% activity at 50°C, 100% activity at 55°C and less than 60% activity at 60°C | Aspergillus versicolor |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme is relatively unstable at 50°C (within 1 h its activity declines to ca 6%) | Aspergillus versicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.13 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | recombinant enzyme, at pH 5.0 and 25°C | Aspergillus versicolor | |
35.3 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | wild type enzyme, at pH 5.0 and 25°C | Aspergillus versicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
recombinant enzyme | Aspergillus versicolor |
6 | - |
wild type enzyme | Aspergillus versicolor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | the recombinant enzyme shows more than 70% activity between pH 3.0 and 6.0. The wild type enzyme shows more than 80% activity between pH 3.0 and 7.0 | Aspergillus versicolor |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0096 | - |
recombinant enzyme, at pH 5.0 and 25°C | Aspergillus versicolor | 1,2,3-tris-[1-(2-acetamido-2-deoxy-beta-D-galactopyranosyl-(1->4)-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-1H-1,2,3-triazol-4-yl)-2-ethyloxy]benzene | |
0.055 | - |
recombinant enzyme, at pH 5.0 and 25°C | Aspergillus versicolor | beta-D-GalNAc-(1->4)beta-D-GlcNAc | |
0.153 | - |
recombinant enzyme, at pH 5.0 and 25°C | Aspergillus versicolor | lactose |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
45.2 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | recombinant enzyme, at pH 5.0 and 25°C | Aspergillus versicolor | |
160.5 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | wild type enzyme, at pH 5.0 and 25°C | Aspergillus versicolor |