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Literature summary for 3.2.1.52 extracted from

  • Bojarova, P.; Kulik, N.; Slamova, K.; Hubalek, M.; Kotik, M.; Cvacka, J.; Pelantova, H.; Kren, V.
    Selective beta-N-acetylhexosaminidase from Aspergillus versicolor-a tool for producing bioactive carbohydrates (2019), Appl. Microbiol. Biotechnol., 103, 1737-1753 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain KM71H Aspergillus versicolor

Inhibitors

Inhibitors Comment Organism Structure
1,2,3-tris-[1-(2-acetamido-2-deoxy-beta-D-galactopyranosyl-(1->4)-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-1H-1,2,3-triazol-4-yl)-2-ethyloxy]benzene
-
Aspergillus versicolor
beta-D-GalNAc-(1->4)beta-D-GlcNAc
-
Aspergillus versicolor
lactose
-
Aspergillus versicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide recombinant enzyme, at pH 5.0 and 25°C Aspergillus versicolor
0.22
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 5.0 and 25°C Aspergillus versicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
MALDI TOF mass spectrometry Aspergillus versicolor

Organism

Organism UniProt Comment Textmining
Aspergillus versicolor A0A1L9P8I4
-
-
Aspergillus versicolor CCF 2491 A0A1L9P8I4
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Aspergillus versicolor

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography Aspergillus versicolor

Storage Stability

Storage Stability Organism
4°C, purified and concentrated enzyme in McIlvaine buffer pH 5.0 conserved with 0.03% (w/v) NaN3, ca 6 months, less than 20% loss of activity Aspergillus versicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
Aspergillus versicolor 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose
-
?
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
Aspergillus versicolor CCF 2491 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Aspergillus versicolor 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Aspergillus versicolor CCF 2491 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
?

Subunits

Subunits Comment Organism
? x * 56872, catalytic domain, calculated from amino acid sequence Aspergillus versicolor
heterodimer 1 * 78000 + 1 * 64000, SDS-PAGE Aspergillus versicolor

Synonyms

Synonyms Comment Organism
Hex
-
Aspergillus versicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Aspergillus versicolor

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 the recombinant enzyme shows more than 80% activity between 40 and 60°C. The wild type enzyme shows about 65% activity at 45°C, 90% activity at 50°C, 100% activity at 55°C and less than 60% activity at 60°C Aspergillus versicolor

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
the enzyme is relatively unstable at 50°C (within 1 h its activity declines to ca 6%) Aspergillus versicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.13
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide recombinant enzyme, at pH 5.0 and 25°C Aspergillus versicolor
35.3
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 5.0 and 25°C Aspergillus versicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
recombinant enzyme Aspergillus versicolor
6
-
wild type enzyme Aspergillus versicolor

pH Range

pH Minimum pH Maximum Comment Organism
3 7 the recombinant enzyme shows more than 70% activity between pH 3.0 and 6.0. The wild type enzyme shows more than 80% activity between pH 3.0 and 7.0 Aspergillus versicolor

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0096
-
recombinant enzyme, at pH 5.0 and 25°C Aspergillus versicolor 1,2,3-tris-[1-(2-acetamido-2-deoxy-beta-D-galactopyranosyl-(1->4)-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-1H-1,2,3-triazol-4-yl)-2-ethyloxy]benzene
0.055
-
recombinant enzyme, at pH 5.0 and 25°C Aspergillus versicolor beta-D-GalNAc-(1->4)beta-D-GlcNAc
0.153
-
recombinant enzyme, at pH 5.0 and 25°C Aspergillus versicolor lactose

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
45.2
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide recombinant enzyme, at pH 5.0 and 25°C Aspergillus versicolor
160.5
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 5.0 and 25°C Aspergillus versicolor