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Literature summary for 3.2.1.52 extracted from
- Mammalian cells contain a second nucleocytoplasmic hexosaminidase (2009), Biochem. J., 419, 83-90.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)pLysS cells | Homo sapiens |
| expressed in Escherichia coli BL21(DE3)pLysS cells. Plasmid encoding an N-terminally Myc-tagged form of the full-length murine hexosaminidase transfected into murine NIH 3T3 fibroblast cells. Myc-tagged protein transfected into COS cells | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | low inhibition with 2-acetamido-1,2-dideoxynojirimycin, streptozotocin, N-acetylglucosamine and N-acetylgalactosamine | Mus musculus | |
| N-acetylcastanospermine | 30% with 1 mM inhibitor and 2.5 mM substrate | Mus musculus |  |
| O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino N-phenylcarbamate | 60% inhibition with 1 mM inhibitor and 2.5 mM substrate | Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
4-nitrophenyl beta-N-acetyl-D-galactosaminide | - |
Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Mus musculus | 5737 | - |
| nucleus | - |
Mus musculus | 5634 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
Western blot analysis | Homo sapiens |
| 55000 | 62000 | Western blot analysis, shorter and longer form | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | Q3U4H6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| atrium | - |
Mus musculus | - |
| HEK-293 cell | - |
Homo sapiens | - |
| kidney | - |
Mus musculus | - |
| liver | - |
Mus musculus | - |
| lung | - |
Mus musculus | - |
| additional information | ventricle | Mus musculus | - |
| soleus | - |
Mus musculus | - |
| tibialis anterior | - |
Mus musculus | - |
| uterus | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O | - |
Homo sapiens | 4-nitrophenol + N-acetyl-beta-D-galactosamine | - |
? | |
| 4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O | - |
Mus musculus | 4-nitrophenol + N-acetyl-beta-D-galactosamine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HEXDC | - |
Homo sapiens |
| HEXDC | - |
Mus musculus |
| hexosaminidase D | - |
Homo sapiens |
| hexosaminidase D | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Mus musculus |
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Release 2023.1 (January 2023)
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