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Literature summary for 3.2.1.52 extracted from

  • Souza, D.S.; Grossi-de-Sa, M.F.; Silva, L.P.; Franco, O.L.; Gomes-Junior, J.E.; Oliveira, G.R.; Rocha, T.L.; Magalhaes, C.P.; Marra, B.M.; Grossi-de-Sa, M.; Romano, E.; de Sa, C.M.; Kombrink, E.; Jimenez, A.V.; Abreu, L.R.
    Identification of a novel beta-N-acetylhexosaminidase (Pcb-NAHA1) from marine Zoanthid Palythoa caribaeorum (Cnidaria, Anthozoa, Zoanthidea) (2008), Protein Expr. Purif., 58, 61-69.
    View publication on PubMed
Search for more literature for 3.2.1.52

Activating Compound

Activating Compound Comment Organism Structure
chondroitin 4-sulfate 0.025 mg/ml, 1.19fold activation Palythoa caribaeorum
chondroitin 6-sulfate 0.025 mg/ml, 1.35fold activation Palythoa caribaeorum
Phenylmethylsulfonylfluoride 1 mM, 1.46fold activation Palythoa caribaeorum

Inhibitors

Inhibitors Comment Organism Structure
D-fructose 3 mM, 26% inhibition Palythoa caribaeorum
D-mannose 3 mM, 19% inhibition Palythoa caribaeorum
fucoidan 0.025 mg/ml, 19% inhibition Palythoa caribaeorum
HgCl2 1 mM, complete inhibition Palythoa caribaeorum
iodoacetamide 1 mM, 16% inhibition Palythoa caribaeorum
lactose 3 mM, 20% inhibition Palythoa caribaeorum
maltose 3 mM, 33% inhibition Palythoa caribaeorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.53
-
 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranose
-
 Palythoa caribaeorum

Metals/Ions

Metals/Ions Comment Organism Structure
BaCl2 1 mM, 1.4fold activation Palythoa caribaeorum
MgCl2 1 mM, 1.17fold activation Palythoa caribaeorum
Na2SeO4 1 mM, 1.45fold activation Palythoa caribaeorum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 25000, SDS-PAGE Palythoa caribaeorum

Organism

Organism UniProt Comment Textmining
Palythoa caribaeorum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Palythoa caribaeorum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 57.9 U/mg. One unit of enzyme activity was defined as the amount of enzyme which released 0.01 absorbance at 405 nm Palythoa caribaeorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranose + H2O
-
 Palythoa caribaeorum 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose
-
 ?
4-nitrophenyl-2-(acetamino)-2-deoxy-beta-D-glucopyranoside + H2O preferentially hydrolyzed Palythoa caribaeorum 4-nitrophenol + N-acetyl-D-glucosamine
-
 ?
additional information no activity with chitin azure Palythoa caribaeorum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Palythoa caribaeorum

Synonyms

Synonyms Comment Organism
Pcb-NAHA1
-
 Palythoa caribaeorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 60
-
 Palythoa caribaeorum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 70 37°C: about 60% of maximal activity, 70°C: about 25% of maximal activity Palythoa caribaeorum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.61
-
 4-nitrophenyl-2-(acetamino)-2-deoxy-beta-D-glucopyranoside
-
 Palythoa caribaeorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Palythoa caribaeorum

pH Range

pH Minimum pH Maximum Comment Organism
4 8.5 pH 4.0: about 35% of maximal activity, pH 5.5: about 35% of maximal activity, pH 8.5: about 50% of maximal activity Palythoa caribaeorum