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Literature summary for 3.2.1.52 extracted from
- Enzymatic properties and subcellular localization of Arabidopsis beta-N-acetylhexosaminidases (2007), Plant Physiol., 145, 5-16.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Sf21 cells. HEXO fluorescent protein fusions transiently expressed in Nicotiana benthamiana | Arabidopsis thaliana |
| HEXO1 lacking its 32 N-terminal amino acids is cloned into baculovirus transfer vector pVTBac-His-1 and is expressed in Sf21. HEXO fluorescent protein fusions transiently expressed in Nicotiana benthamiana | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
Arabidopsis thaliana |  |
| 1.2 | - |
p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
Arabidopsis thaliana | |
| 2.2 | - |
p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Arabidopsis thaliana | 5886 | - |
| vacuole | HEXO1 participates in N-glycan trimming in the vacuole | Arabidopsis thaliana | 5773 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
x * 61000, calculated from sequence | Arabidopsis thaliana |
| 61800 | - |
x * 61800, calculated from sequence | Arabidopsis thaliana |
| 64000 | - |
x * 64000, SDS-PAGE | Arabidopsis thaliana |
| 65000 | - |
x * 65000, SDS-PAGE | Arabidopsis thaliana |
| 65600 | - |
x * 65600, calculated from sequence | Arabidopsis thaliana |
| 68000 | - |
x * 68000, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Arabidopsis thaliana | HEXO1 participates in N-glycan trimming in the vacuole | ? | - |
? | |
| additional information | Arabidopsis thaliana | HEXO2 is responsible for the processing of N-glycans present on secretory glycoproteins | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | A7WM73 | - |
- |
| Arabidopsis thaliana | Q8L7S6 | - |
- |
| Arabidopsis thaliana | Q9SYK0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Arabidopsis thaliana | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
? | |
| 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Arabidopsis thaliana | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranose | - |
? | |
| 4-methylumbelliferyl-6-sulfo-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Arabidopsis thaliana | 4-methylumbelliferone + 6-sulfo-2-acetamido-2-deoxy-beta-D-glucopyranose | - |
? | |
| 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O | - |
Arabidopsis thaliana | 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-galactopyranose | - |
? | |
| 4-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Arabidopsis thaliana | 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose | - |
? | |
| additional information | HEXO1 participates in N-glycan trimming in the vacuole | Arabidopsis thaliana | ? | - |
? | |
| additional information | HEXO2 is responsible for the processing of N-glycans present on secretory glycoproteins | Arabidopsis thaliana | ? | - |
? | |
| additional information | HEXO1 is capable of removing terminal GlcNAc residues from the alpha1,3- and alpha1,6-mannosyl branches of biantennary N-glycans without any strict branch preference with N-glycan substrates. HEXO1 displayed a much higher specific activity than HEXO2 and HEXO3 | Arabidopsis thaliana | ? | - |
? | |
| p-nitrophenyl-2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O | - |
Arabidopsis thaliana | p-nitrophenol + 2-acetamido-2-deoxy-beta-D-galactopyranose | - |
? | |
| p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Arabidopsis thaliana | p-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose | - |
? | |
| pyridylaminated chitobiose + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
| pyridylaminated chitotriose + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, SDS-PAGE | Arabidopsis thaliana |
| ? | x * 64000, SDS-PAGE | Arabidopsis thaliana |
| ? | x * 68000, SDS-PAGE | Arabidopsis thaliana |
| ? | x * 61000, calculated from sequence | Arabidopsis thaliana |
| ? | x * 61800, calculated from sequence | Arabidopsis thaliana |
| ? | x * 65600, calculated from sequence | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| At1g65600/F5I14_13 | - |
Arabidopsis thaliana |
| F3F20.4 protein | - |
Arabidopsis thaliana |
| hEXO1 | - |
Arabidopsis thaliana |
| HEXO2 | - |
Arabidopsis thaliana |
| HEXO3 | - |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
hydrolysis p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside | Arabidopsis thaliana |
| 5 | - |
hydrolysis p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside | Arabidopsis thaliana |
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