Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 5 mM, 93% inhibition | Zea mays | |
CuCl2 | 5 mM, 37% inhibition | Zea mays | |
FeCl3 | 5 mM, 90% inhibition | Zea mays | |
HgCl2 | 5 mM, 96% inhibition | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
AlCl3 | 5 mM, activation to 124% | Zea mays | |
CaCl2 | 5 mM, activation to 116% | Zea mays | |
KCl | 5 mM, activation to 111% | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
gel filtration | Zea mays |
70000 | - |
1 * 70000, SDS-PAGE | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
L. cv. Snowdent 110 | - |
Purification (Comment) | Organism |
---|---|
- |
Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Zea mays | - |
scutellum | - |
Zea mays | - |
seed | substantial activity detected in dry seed. Activity increases before germination but exclusively in the embryo | Zea mays | - |
seedling | most of the activity is found in the scutellum, and lower levels in shoots and roots | Zea mays | - |
shoot | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | release of GlcNAc after 24 h | Zea mays | GlcNAc + ? | - |
? | |
additional information | no activity with oligo-sugar I, oligo-sugar II and ovalbumin | Zea mays | ? | - |
? | |
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O | - |
Zea mays | N-acetyl-D-glucosamine + ? | - |
? | |
N,N',N'',N'''-tetraacetylchitotetraose + H2O | - |
Zea mays | N-acetyl-D-glucosamine + ? | - |
? | |
N,N',N''-triacetylchitotriose + H2O | - |
Zea mays | N-acetyl-D-glucosamine + N,N'-diacetylchiotobiose | - |
? | |
N,N'-diacetylchitobiose + H2O | - |
Zea mays | deacetylated chitooligosaccharides + acetate | - |
? | |
p-nitrophenyl-N-acetyl-beta-D-galactosaminide + H2O | 7.39% of the activity with (GlcNAc)2 | Zea mays | p-nitrophenol + N-acetyl-beta-D-galactosamine | - |
? | |
p-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O | 5.95% of the activity with (GlcNAc)2 | Zea mays | p-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 70000, SDS-PAGE | Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
reaction with (GlcNAc)2 | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6 | 50% of maximal activcity at pH 3.5 and pH 6.0 | Zea mays |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Zea mays | chromatofocusing | - |
6 |