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Literature summary for 3.2.1.52 extracted from
- Purification and characterization of an N-acetylglucosaminidase produced by a Trichoderma harzianum strain which controls Crinipellis perniciosa (2004), Appl. Microbiol. Biotechnol., 64, 70-75.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AlCl3 | 1 mM, 24% inhibition | Trichoderma harzianum |  |
| CaCl2 | 1 mM, 30% inhibition | Trichoderma harzianum | |
| CoCl2 | 1 mM, complete inhibition | Trichoderma harzianum | |
| CuSO4 | 1 mM, 36% inhibition | Trichoderma harzianum | |
| FeCl3 | 1 mM, complete inhibition | Trichoderma harzianum | |
| glucose | 1 mM, 35% inhibition | Trichoderma harzianum | |
| MnCl2 | 1 mM, complete inhibition | Trichoderma harzianum | |
| ZnSO4 | 1 mM, 77% inhibition | Trichoderma harzianum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00806 | - |
p-nitrophenyl-N-acetylglucosaminide | pH 6.0, 37°C | Trichoderma harzianum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 36000, SDS-PAGE | Trichoderma harzianum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma harzianum | - |
isolate 1051 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trichoderma harzianum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-N-acetylglucosaminide + H2O | - |
Trichoderma harzianum | p-nitrophenol + N-acetylglucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000, SDS-PAGE | Trichoderma harzianum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Trichoderma harzianum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 30°C: about 55% of maximal activity, 50-60°C: maximal activity, 70°C: less than 10% of maximal activity | Trichoderma harzianum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
30 min, about 30% loss of activity. About 40% loss of acticity after 12 h | Trichoderma harzianum |
| 50 | - |
about 50% loss of activity after 1 h. About 85% loss of activity after 12 h | Trichoderma harzianum |
| 60 | - |
complete loss of activity after 30 min | Trichoderma harzianum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
hydrolysis of p-nitrophenyl-N-acetylglucosaminide | Trichoderma harzianum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | pH 3.0: about 55% of maximal activity, pH 6.0: about 70% of maximal activity, hydrolysis of p-nitrophenyl-N-acetylglucosaminide | Trichoderma harzianum |
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Release 2023.1 (January 2023)
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