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Literature summary for 3.2.1.52 extracted from

  • Dimond, R.L.; Loomis, W.F.
    Vegetative isozyme of N-acetylglucosaminidase in Dictyostelium discoideum (1974), J. Biol. Chem., 249, 5628-5632.
    View publication on PubMed
Search for more literature for 3.2.1.52

Inhibitors

Inhibitors Comment Organism Structure
Ag+ minor isozyme: 2% of activity remains with 5 mM, major isozyme: 38% of activity remains with 2 mM Dictyostelium discoideum
Cu2+ minor isozyme: 19% of activity remains with 10 mM, major isozyme: 47% of activity remains with 10 mM Dictyostelium discoideum
Hg2+ minor isozyme: 5% of activity remains with 0.01 mM, major isozyme: 53% of activity remains with 0.01 mM Dictyostelium discoideum
Zn2+ minor isozyme: 8% of activity remains with 5 mM, major isozyme: 40% of activity remains with 2 mM Dictyostelium discoideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Dictyostelium discoideum
1.5
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside extract of wild type strain, purified major and minor isozyme Dictyostelium discoideum
7.8
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside extract of mutant strains Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Dictyostelium discoideum
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
-
-
Dictyostelium discoideum A3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
stage-specific enzyme: 2200fold, vegetative enzyme: 1400fold Dictyostelium discoideum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4
-
 vegetative enzyme Dictyostelium discoideum
262
-
 stage-specific enzyme Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
 Dictyostelium discoideum N-acetylglucosamine
-
 ?
chitin + H2O
-
 Dictyostelium discoideum A3 N-acetylglucosamine
-
 ?
chondroitinsulfate C + H2O
-
 Dictyostelium discoideum ?
-
 ?
hyaluronic acid + H2O
-
 Dictyostelium discoideum N-acetylglucosamine + ?
-
 ?
hyaluronic acid + H2O
-
 Dictyostelium discoideum A3 N-acetylglucosamine + ?
-
 ?
murein + H2O bacterial cell wall murein, activity against this substrate at pH 3.5 and not 5.0 Dictyostelium discoideum ?
-
 ?
murein + H2O bacterial cell wall murein, activity against this substrate at pH 3.5 and not 5.0 Dictyostelium discoideum A3 ?
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Dictyostelium discoideum N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Dictyostelium discoideum 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Dictyostelium discoideum A3 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Dictyostelium discoideum 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Dictyostelium discoideum A3 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 major peak: half-life of 17 min at pH 5.0, minor peak: half-life of more than 100 min at pH 5.0 Dictyostelium discoideum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 extract of mutant strains Dictyostelium discoideum
5
-
 extract of wild type strain Dictyostelium discoideum