Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | with 1 mM 43% of N-acetylgalactosaminidase and 59% of N-acetylglucosaminidase activity remains | Trichoderma harzianum | |
Hg2+ | with 1 mM 84% of N-acetylgalactosaminidase and 90% of N-acetylglucosaminidase activity remains | Trichoderma harzianum | |
N-acetyl-D-glucosamine | inhibits N-acetylgalactosaminidase activity | Trichoderma harzianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Trichoderma harzianum | |
27 | - |
pyridylamino-glycoside B | - |
Trichoderma harzianum | |
30 | - |
pyridylamino-glycoside C | - |
Trichoderma harzianum | |
440 | - |
pyridylamino-glycoside A | - |
Trichoderma harzianum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Trichoderma harzianum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
SDS-PAGE | Trichoderma harzianum |
150000 | - |
gel filtration | Trichoderma harzianum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | Trichoderma harzianum | - |
N-acetyl-D-hexosamines | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma harzianum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
13.2fold to homogeneity | Trichoderma harzianum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Trichoderma harzianum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000085 | - |
substrate: pyridylamino-glycoside A | Trichoderma harzianum |
0.0014 | - |
substrate: pyridylamino-glycoside C | Trichoderma harzianum |
0.91 | - |
substrate: pyridylamino-glycoside B | Trichoderma harzianum |
102 | - |
- |
Trichoderma harzianum |
Storage Stability | Organism |
---|---|
-20°C | Trichoderma harzianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hexa-N-acetylchitohexaose + H2O | - |
Trichoderma harzianum | ? | - |
? | |
N,N',N'',N'''-tetraacetylchitotetraose + 3 H2O | - |
Trichoderma harzianum | 4 N-acetyl-D-glucosamine | - |
? | |
N,N'-diacetylchitobiose + H2O | - |
Trichoderma harzianum | N-acetyl-D-glucosamine | - |
? | |
N-acetyl-beta-D-hexosaminides + H2O | - |
Trichoderma harzianum | N-acetyl-D-hexosamines | - |
? | |
p-nitrophenyl tetra-N-acetylchitotetraoside + H2O | - |
Trichoderma harzianum | N-acetylglucosamine | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Trichoderma harzianum | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Trichoderma harzianum | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
penta-N-acetylchitopentaose + H2O | - |
Trichoderma harzianum | ? | - |
? | |
pyridylamino-glycoside A + H2O | pyridylamino-GlcNAcbeta(1-4)GlcNAc | Trichoderma harzianum | ? | - |
? | |
pyridylamino-glycoside B + H2O | pyridylamino-GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | Trichoderma harzianum | ? | - |
? | |
pyridylamino-glycoside C + H2O | pyridylamino-GlcNAcbeta(1-3)GalNAc(1-4)Glc | Trichoderma harzianum | ? | - |
? | |
tri-N-acetylchitotriose + H2O | - |
Trichoderma harzianum | N-acetyl-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 69000 | Trichoderma harzianum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
at pH 5.2 | Trichoderma harzianum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
below: completely stable for 30 min | Trichoderma harzianum |
65 | - |
over 50% of activity remains after 30 min | Trichoderma harzianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 5.5 | - |
Trichoderma harzianum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | 10 | not less than 80% of activity remains, at 5°C for 1 h | Trichoderma harzianum |
3 | 9 | not less than 80% of activity remains, at 37°C for 1 h | Trichoderma harzianum |