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Literature summary for 3.2.1.52 extracted from

  • Koga, K.; Iwamoto, Y.; Sakamoto, H.; Hatano, K.; Sano, M.; Kato, I.
    Purification and characterization of beta-N-acetylhexosaminidase from Trichoderma harzianum (1991), Agric. Biol. Chem., 55, 2817-2823.
    View publication on PubMed
Search for more literature for 3.2.1.52

Inhibitors

Inhibitors Comment Organism Structure
Ag+ with 1 mM 43% of N-acetylgalactosaminidase and 59% of N-acetylglucosaminidase activity remains Trichoderma harzianum
Hg2+ with 1 mM 84% of N-acetylgalactosaminidase and 90% of N-acetylglucosaminidase activity remains Trichoderma harzianum
N-acetyl-D-glucosamine inhibits N-acetylgalactosaminidase activity Trichoderma harzianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Trichoderma harzianum
27
-
 pyridylamino-glycoside B
-
 Trichoderma harzianum
30
-
 pyridylamino-glycoside C
-
 Trichoderma harzianum
440
-
 pyridylamino-glycoside A
-
 Trichoderma harzianum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Trichoderma harzianum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
 SDS-PAGE Trichoderma harzianum
150000
-
 gel filtration Trichoderma harzianum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Trichoderma harzianum
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
13.2fold to homogeneity Trichoderma harzianum

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Trichoderma harzianum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000085
-
 substrate: pyridylamino-glycoside A Trichoderma harzianum
0.0014
-
 substrate: pyridylamino-glycoside C Trichoderma harzianum
0.91
-
 substrate: pyridylamino-glycoside B Trichoderma harzianum
102
-
-
 Trichoderma harzianum

Storage Stability

Storage Stability Organism
-20°C Trichoderma harzianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hexa-N-acetylchitohexaose + H2O
-
 Trichoderma harzianum ?
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + 3 H2O
-
 Trichoderma harzianum 4 N-acetyl-D-glucosamine
-
 ?
N,N'-diacetylchitobiose + H2O
-
 Trichoderma harzianum N-acetyl-D-glucosamine
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Trichoderma harzianum N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl tetra-N-acetylchitotetraoside + H2O
-
 Trichoderma harzianum N-acetylglucosamine
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Trichoderma harzianum 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Trichoderma harzianum 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
penta-N-acetylchitopentaose + H2O
-
 Trichoderma harzianum ?
-
 ?
pyridylamino-glycoside A + H2O pyridylamino-GlcNAcbeta(1-4)GlcNAc Trichoderma harzianum ?
-
 ?
pyridylamino-glycoside B + H2O pyridylamino-GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc Trichoderma harzianum ?
-
 ?
pyridylamino-glycoside C + H2O pyridylamino-GlcNAcbeta(1-3)GalNAc(1-4)Glc Trichoderma harzianum ?
-
 ?
tri-N-acetylchitotriose + H2O
-
 Trichoderma harzianum N-acetyl-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 69000 Trichoderma harzianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 at pH 5.2 Trichoderma harzianum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 below: completely stable for 30 min Trichoderma harzianum
65
-
 over 50% of activity remains after 30 min Trichoderma harzianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5.5
-
 Trichoderma harzianum

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 10 not less than 80% of activity remains, at 5°C for 1 h Trichoderma harzianum
3 9 not less than 80% of activity remains, at 37°C for 1 h Trichoderma harzianum