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Literature summary for 3.2.1.52 extracted from
- Purification and characterization of beta-N-acetylhexosaminidase from the ascidian, Halocynthia roretzi (1983), J. Biochem., 93, 847-855.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | with 0.1 mM 63% of activity remains | Halocynthia roretzi |  |
| Cu2+ | with 10 mM 47% of activity remains | Halocynthia roretzi | |
| Hg2+ | concentration of 0.1 mM results in total loss of activity | Halocynthia roretzi | |
| sodium taurodeoxycholate | - |
Halocynthia roretzi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.87 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | - |
Halocynthia roretzi | |
| 1.92 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Halocynthia roretzi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
SDS-PAGE | Halocynthia roretzi |
| 170000 | - |
at pH 7.0, gel filtration | Halocynthia roretzi |
| 330000 | - |
at pH 4.5, gel filtration | Halocynthia roretzi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Halocynthia roretzi | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halocynthia roretzi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 820fold | Halocynthia roretzi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| viscus | - |
Halocynthia roretzi | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 214.1 | - |
- |
Halocynthia roretzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| asialo GM2 ganglioside + H2O | - |
Halocynthia roretzi | lactosylceramide | - |
? | |
| globoside I + H2O | - |
Halocynthia roretzi | trihexaosylceramide + GalNAc | - |
? | |
| GM2 ganglioside + H2O | - |
Halocynthia roretzi | GM3 ganglioside + N-acetyl-beta-D-galactosamine | - |
? | |
| N-acetyl-beta-D-hexosaminides + H2O | - |
Halocynthia roretzi | N-acetyl-D-hexosamines | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Halocynthia roretzi | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Halocynthia roretzi | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable for 30 min | Halocynthia roretzi |
| 50 | - |
loses 30% of activity after 30 min | Halocynthia roretzi |
| 60 | - |
loses almost all activity after 5 min | Halocynthia roretzi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | Halocynthia roretzi |
| 4.2 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Halocynthia roretzi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
active | Halocynthia roretzi |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7.5 | stable | Halocynthia roretzi |
| 7 | - |
inactive | Halocynthia roretzi |
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