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Literature summary for 3.2.1.52 extracted from

  • Yamamoto, K.; Lee, K.M.; Kumagai, H.; Tochikura, T.
    Purification and characterization of beta-N-acetylhexosaminidase from Penicillium oxalicum (1985), Agric. Biol. Chem., 49, 611-619.
No PubMed abstract available
Search for more literature for 3.2.1.52

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 0.05 mM results in significant inhibition Penicillium oxalicum
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside at higher concentrations Penicillium oxalicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.48
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Penicillium oxalicum
1
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Penicillium oxalicum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Penicillium oxalicum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 2 * 68000, SDS-PAGE Penicillium oxalicum
141000
-
 sedimentation equilibrium Penicillium oxalicum
143000
-
 gel filtration Penicillium oxalicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Penicillium oxalicum
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium oxalicum
-
 IFO 5748
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Penicillium oxalicum

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Penicillium oxalicum

Reaction

Reaction Comment Organism Reaction ID
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose exo-type reaction Penicillium oxalicum
a

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Penicillium oxalicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
126
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Penicillium oxalicum
186
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Penicillium oxalicum

Storage Stability

Storage Stability Organism
-20°C, enzyme preparation concentrated by ultrafiltration Penicillium oxalicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycopeptide + H2O glycopeptide obtained by modification of fetuin Penicillium oxalicum N-acetyl-D-glucosamine
-
 ?
N,N'-diacetylchitobiose + H2O
-
 Penicillium oxalicum N-acetyl-D-glucosamine
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Penicillium oxalicum N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Penicillium oxalicum 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Penicillium oxalicum 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
tri-N-acetylchitotriose + H2O
-
 Penicillium oxalicum N-acetyl-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 68000, SDS-PAGE Penicillium oxalicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Penicillium oxalicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable for 10 min Penicillium oxalicum
65
-
 complete inactivation within 10 min Penicillium oxalicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 4.5
-
 Penicillium oxalicum

pH Range

pH Minimum pH Maximum Comment Organism
2.5 5 active within this range Penicillium oxalicum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 stable within this range Penicillium oxalicum