Activating Compound | Comment | Organism | Structure |
---|---|---|---|
low molecular weight protein | restores the enzyme's ability to hydrolyze GM2 in high ionic strength | Mugil cephalus |
General Stability | Organism |
---|---|
high purification results in great loss of activity | Mugil cephalus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
high ionic strength | above 0.1 no cleavage of GM2 | Mugil cephalus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
beta-hexosaminidase II + III | Mugil cephalus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | Mugil cephalus | - |
N-acetyl-D-hexosamines | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mugil cephalus | - |
striped mullet | - |
Purification (Comment) | Organism |
---|---|
beta-hexosaminidase II: 202fold , beta-hexosaminidase III: 426fold, still very heterogenous | Mugil cephalus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
roe | - |
Mugil cephalus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.3 | - |
- |
Mugil cephalus |
Storage Stability | Organism |
---|---|
-20°C, in 10 mM sodium acetate buffer pH 4.0, very unstable | Mugil cephalus |
-20°C, in 5-50 mM sodium citrate buffer pH 4.0, very stable, even after repeated freeze-thawing | Mugil cephalus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GM2 ganglioside + H2O | - |
Mugil cephalus | GM3 ganglioside + N-acetyl-beta-D-galactosamine | - |
? | |
N-acetyl-beta-D-hexosaminides + H2O | - |
Mugil cephalus | N-acetyl-D-hexosamines | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Mugil cephalus | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Mugil cephalus | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
Sda oligosaccharide + H2O | - |
Mugil cephalus | N-acetyl-D-galactosamine | - |
? | |
SRO oligosaccharide + H2O | - |
Mugil cephalus | N-acetyl-D-galactosamine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Mugil cephalus |