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Literature summary for 3.2.1.52 extracted from

  • Wheeler, P.R.; Bharadwaj, V.P.; Gregory, D.
    N-Acetyl-beta-glucosaminidase, beta-glucuronidase and acid phosphatase in Mycobacterium leprae (1982), J. Gen. Microbiol., 128, 1063-1071.
    View publication on PubMed
Search for more literature for 3.2.1.52

Activating Compound

Activating Compound Comment Organism Structure
acetate untreated, concentration of 50 mM: activity reaches 170% Mycobacterium leprae

Inhibitors

Inhibitors Comment Organism Structure
7-diazonaphthalene-1,3-disulfonic acid extract: 45% of activity remains Mycobacterium leprae
acetate with 50 mM 45% of activity remains Armadillo officinalis
N-acetyl-beta-D-glucosaminolactone with 0.02 30% of activity remains Armadillo officinalis
N-acetyl-beta-D-glucosaminolactone untreated: with 0.02 mM 65% of activity remains, treated with 7-diazonaphtalene-1,3-disulfonic acid: with 0.02 mM 70% of activity remains Mycobacterium leprae
NaOH unbroken bacteria: total inhibition with 1 M, extract: with 1 M 9% of activity remains Mycobacterium leprae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mycolicibacterium phlei 16020
-
membrane
-
 Mycobacterium tuberculosis variant microti 16020
-
membrane
-
 Mycobacterium leprae 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Mycolicibacterium phlei
-
 N-acetyl-D-hexosamines
-
 ?
N-acetyl-beta-D-hexosaminides + H2O Mycobacterium tuberculosis variant microti
-
 N-acetyl-D-hexosamines
-
 ?
N-acetyl-beta-D-hexosaminides + H2O Mycobacterium leprae
-
 N-acetyl-D-hexosamines
-
 ?
N-acetyl-beta-D-hexosaminides + H2O Armadillo officinalis
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Armadillo officinalis
-
-
-
Mycobacterium leprae
-
 from armadillo liver
-
Mycobacterium tuberculosis variant microti
-
-
-
Mycolicibacterium phlei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
30fold Mycobacterium leprae
30fold Armadillo officinalis

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Armadillo officinalis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Mycolicibacterium phlei
additional information
-
-
 Mycobacterium tuberculosis variant microti
additional information
-
-
 Mycobacterium leprae
additional information
-
-
 Armadillo officinalis

Storage Stability

Storage Stability Organism
-80°C, crude cell-free extract, 0.25-2.0 mg protein/ml Mycolicibacterium phlei
-80°C, crude cell-free extract, 0.25-2.0 mg protein/ml Mycobacterium tuberculosis variant microti
-80°C, crude cell-free extract, 0.25-2.0 mg protein/ml Mycobacterium leprae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O
-
 Mycolicibacterium phlei N-acetyl-D-hexosamines
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Mycobacterium tuberculosis variant microti N-acetyl-D-hexosamines
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Mycobacterium leprae N-acetyl-D-hexosamines
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Armadillo officinalis N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Mycolicibacterium phlei 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Mycobacterium tuberculosis variant microti 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Mycobacterium leprae 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Armadillo officinalis 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5 9 inactivation at high pH like 8.5-9.0 Armadillo officinalis