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Literature summary for 3.2.1.52 extracted from
- Neutral beta-N-acetylhexosaminidases of rat brain. Purification and enzymatic and immunological characterization (1983), J. Biol. Chem., 258, 6991-6999.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | N-acetylgalactosaminidase, stimulation up to 4fold, stimulation of GM2-hydrolysis, effect on N-acetylglucosaminidase only slight | Rattus norvegicus | |
| dithiothreitol | stimulates N-acetylglucosaminidase even at low concentrations | Rattus norvegicus |  |
| neutral N-acetylglucosaminidase | stimulates GM2-hydrolysis by N-acetylgalactosaminidase | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-acetyl-D-galactosamine | inhibits N-galactosaminidase | Rattus norvegicus | |
| N-acetyl-D-glucosamine | inhibits N-glucosaminidase | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.42 | - |
4-methylumbelliferyl beta-N-acetylgalactosaminide | N-acetylgalactosaminidase | Rattus norvegicus | |
| 0.45 | - |
4-methylumbelliferyl beta-N-acetylgalactosaminide | N-acetylglucosaminidase | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
neutral N-acetylgalactosaminidase, SDS-PAGE | Rattus norvegicus |
| 82000 | - |
neutral N-acetylgalactosaminidase, sucrose density gradient centrifugation | Rattus norvegicus |
| 88000 | - |
neutral N-acetylglucosaminidase, sucrose density gradient centrifugation | Rattus norvegicus |
| 130000 | - |
neutral N-acetylglucosaminidase, SDS-PAGE | Rattus norvegicus |
| 210000 | - |
neutral N-acetylgalactosaminidase, gel filtration | Rattus norvegicus |
| 525000 | - |
neutral N-acetylglucosaminidase, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Rattus norvegicus | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| N-acetylhexosaminidase: 63fold | Rattus norvegicus |
| neutral N-acetylgalactosaminidase, 2000-2500fold to homogeneity | Rattus norvegicus |
| neutral N-acetylglucosaminidase, 300-330fold, 85-90% pure | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl beta-N-acetylgalactosaminide + H2O | - |
Rattus norvegicus | 4-methylumbelliferone + beta-N-acetylgalactosamine | - |
? | |
| 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O | - |
Rattus norvegicus | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-galactopyranoside | - |
? | |
| asialo GM2 ganglioside + H2O | neutral N-acetylgalactosaminidase | Rattus norvegicus | lactosylceramide | - |
? | |
| globoside + H2O | neutral N-acetylgalactosaminidase | Rattus norvegicus | ? | - |
? | |
| GM2 ganglioside + H2O | neutral N-acetylgalactosaminidase | Rattus norvegicus | GM3 ganglioside + N-acetyl-beta-D-galactosamine | - |
? | |
| N-acetyl-beta-D-hexosaminides + H2O | - |
Rattus norvegicus | N-acetyl-D-hexosamines | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | hydrolyzed by neutral N-acetylgalactosaminidase and to a very small extend by neutral N-acetylglucosaminidase | Rattus norvegicus | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | neutral N-acetylglucosaminidase | Rattus norvegicus | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
N-glucosaminidase: complete loss of activity after 30 min with or without addition of bovine serum albumin, N-galactosaminidase: loss of 20% of activity after 30 min with bovine serum albumin | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
substrate: 4-methylumbelliferyl-beta-N-acetylgalactosaminide, N-acetylglucosaminidase | Rattus norvegicus |
| 6 | - |
substrate: 4-methylumbelliferyl-beta-N-acetylgalactosaminide, N-acetylgalactosaminidase | Rattus norvegicus |
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