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Literature summary for 3.2.1.51 extracted from

  • Megson, Z.A.; Koerdt, A.; Schuster, H.; Ludwig, R.; Janesch, B.; Frey, A.; Naylor, K.; Wilson, I.B.; Stafford, G.P.; Messner, P.; Schaeffer, C.
    Characterization of an alpha-L-fucosidase from the periodontal pathogen Tannerella forsythia (2015), Virulence, 6, 282-292 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.51

Cloned(Commentary)

Cloned (Comment) Organism
the Tffuc1 gene is cloned into pET22-b(C) vector and expressed in Escherichia coli as a C-terminally His6-tagged protein Tannerella forsythia

Inhibitors

Inhibitors Comment Organism Structure
alpha-L-fucose competitive Tannerella forsythia
deoxyfuconojirimycin competitive Tannerella forsythia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 4-nitrophenyl alpha-L-fucopyranoside 22°C, pH 9.0 Tannerella forsythia

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information all the detectable activity is found in the non-membrane associated fraction comprising both the cytoplasmic and periplasmic content Tannerella forsythia
-
-

Organism

Organism UniProt Comment Textmining
Tannerella forsythia G8UMQ6
-
-
Tannerella forsythia ATCC 43037 G8UMQ6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Tannerella forsythia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
 Tannerella forsythia 4-nitrophenol + alpha-L-fucopyranose
-
 ?
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
 Tannerella forsythia ATCC 43037 4-nitrophenol + alpha-L-fucopyranose
-
 ?
additional information TfFuc1 is a unique alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates Tannerella forsythia ?
-
 ?
additional information TfFuc1 is a unique alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates Tannerella forsythia ATCC 43037 ?
-
 ?

Synonyms

Synonyms Comment Organism
bfo_2737
-
 Tannerella forsythia
TfFuc1
-
 Tannerella forsythia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Tannerella forsythia

General Information

General Information Comment Organism
physiological function the biological role of the enzyme may well be in the metabolism of short oligosaccharides in the periplasm, thereby indirectly contributing to the virulence of this organism Tannerella forsythia