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Literature summary for 3.2.1.51 extracted from
- Purification, expression and characterization of a novel alpha-L-fucosidase from a marine bacteria Wenyingzhuangia fucanilytica (2017), Protein Expr. Purif., 129, 9-17 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, 1.2fold activation | Wenyingzhuangia fucanilytica |  |
| EDTA | 1 mM, 1.2fold activation | Wenyingzhuangia fucanilytica | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 6xHis-tagged enzyme is expressed in Escherichia coli | Wenyingzhuangia fucanilytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 1 mM, complete inactivation | Wenyingzhuangia fucanilytica | |
| Co2+ | 1 mM, 61% loss of activity | Wenyingzhuangia fucanilytica | |
| deoxyfuconojirimycin | competitively | Wenyingzhuangia fucanilytica | |
| Hg2+ | 1 mM, complete inactivation | Wenyingzhuangia fucanilytica | |
| Zn2+ | 1 mM, complete inactivation | Wenyingzhuangia fucanilytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.3 | - |
4-nitrophenyl alpha-L-fucopyranoside | pH 7.0, 30°C | Wenyingzhuangia fucanilytica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Wenyingzhuangia fucanilytica | 5622 | - |
| periplasmic space | - |
Wenyingzhuangia fucanilytica | 42597 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 1.2fold activation | Wenyingzhuangia fucanilytica | |
| Mg2+ | 1 mM, 1.2fold activation | Wenyingzhuangia fucanilytica | |
| Mn2+ | 1 mM, 1.2fold activation | Wenyingzhuangia fucanilytica | |
| additional information | the enzyme is not significantly influenced by NaCl | Wenyingzhuangia fucanilytica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65300 | - |
gel filtration | Wenyingzhuangia fucanilytica |
| 67500 | - |
SDS-PAGE | Wenyingzhuangia fucanilytica |
| 67721 | - |
MALDI-TOF-MS | Wenyingzhuangia fucanilytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Wenyingzhuangia fucanilytica | A0A1B1NX89 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Wenyingzhuangia fucanilytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.99 | - |
pH 7.0, 30°C | Wenyingzhuangia fucanilytica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 24 h, approximate 78% of initial activity is retained | Wenyingzhuangia fucanilytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-fucopyranoside + H2O | the enzyme shows a low transglycosylation potential | Wenyingzhuangia fucanilytica | 4-nitrophenol + alpha-L-fucopyranose | - |
? | |
| beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-D-GlcNAc + H2O | slightly hydrolyzed, since fucose monosaccharide is detected after the reaction | Wenyingzhuangia fucanilytica | alpha-L-fucose + beta-D-Gal-(1-3)-D-GlcNAc | - |
? | |
| additional information | the enzyme can hydrolyze the partially degraded fragment of fucoidan. No free fucose can be released from alpha-Fuc-(1->2)-beta-Gal-(1->4)-Glc, beta-Gal-(1->4)-[alpha-Fuc-(1->3)]-Glc or alpha-Fuc-(1->6)-GlcNAc | Wenyingzhuangia fucanilytica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 67500, SDS-PAGE | Wenyingzhuangia fucanilytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Alf1_Wf | - |
Wenyingzhuangia fucanilytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Wenyingzhuangia fucanilytica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 35 | 20°C: about 50% of maximal activity, 35°C: about 50% of maximal activity | Wenyingzhuangia fucanilytica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
12 h, 60% loss of activity | Wenyingzhuangia fucanilytica |
| 35 | - |
complete loss of activity | Wenyingzhuangia fucanilytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Wenyingzhuangia fucanilytica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | pH 5.5: about 40% of maximal activity, pH 8.5: about 35% of maximal activity | Wenyingzhuangia fucanilytica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | stable | Wenyingzhuangia fucanilytica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme can hydrolyze the partially degraded fragment of fucoidan, which suggests that the enzyme may contribute to the final degradation of fucoidan in cells of Wenyingzhuangia fucanilytica | Wenyingzhuangia fucanilytica |
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