Literature summary for 3.2.1.51 extracted from

Venugopal, A.; Sudheer Kumar, C.; Siva Kumar, N.; Swamy, M.J.
Kinetic and biophysical characterization of a lysosomal alpha-L-fucosidase from the fresh water mussel, Lamellidens corrianus (2017), Int. J. Biol. Macromol., 104, 432-441 .

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Inhibitors

Inhibitors	Comment	Organism	Structure
L-fucose	-	Lamellidens corrianus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.85	-	4-methylumbelliferyl alpha-L-fucopyranoside	pH 5.0, 37°C	Lamellidens corrianus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
lysosome	-	Lamellidens corrianus	5764	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
56000	-	SDS-PAGE, non-denaturing PAGE	Lamellidens corrianus
56175	-	mass spectrometric analysis	Lamellidens corrianus

Organic Solvent Stability

Organic Solvent	Comment	Organism
guanidine-HCl	the enzyme unfolds in a broad sigmoidal, cooperative unfolding transition, centered at about 2.2 M guanidinium chloride	Lamellidens corrianus
guanidinium thiocyanate	the enzyme unfolds in a broad sigmoidal, cooperative unfolding transition, centered at about 2.2 M guanidinium thiocyanate	Lamellidens corrianus

Organism

Organism	UniProt	Comment	Textmining
Lamellidens corrianus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Lamellidens corrianus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl alpha-L-fucopyranoside + H2O	-	Lamellidens corrianus	4-methylumbelliferone + alpha-L-fucopyranose	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 56000, SDS-PAGE	Lamellidens corrianus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Lamellidens corrianus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
50	70	50°C: about 60% of maximal activity, 70°C: about 80% of maximal activity	Lamellidens corrianus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.01	-	4-methylumbelliferyl alpha-L-fucopyranoside	pH 5.0, 37°C	Lamellidens corrianus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	6	-	Lamellidens corrianus

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	7	pH 4.0: about 50% of maximal activity, pH 7.0: about 65% of maximal activity	Lamellidens corrianus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1.09	-	L-fucose	pH 5.0, 37°C	Lamellidens corrianus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
2.5	-	pH 5.0, 37°C	Lamellidens corrianus	L-fucose

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Release 2023.1 (January 2023)