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Literature summary for 3.2.1.51 extracted from

  • Liu, S.; Kulinich, A.; Cai, Z.P.; Ma, H.Y.; Du, Y.M.; Lv, Y.M.; Liu, L.; Voglmeir, J.
    The fucosidase-pool of Emticicia oligotrophica Biochemical characterization and transfucosylation potential (2016), Glycobiology, 26, 871-879 .
    View publication on PubMed
Search for more literature for 3.2.1.51

Application

Application Comment Organism
analysis the facile recombinant expression procedure and the peculiar substrate specificities make the enzyme an attractive candidates for bioanalytical applications Emticicia oligotrophica
analysis the facile recombinant expression procedure and the peculiar substrate specificities make these enzymes attractive candidates for bioanalytical applications Emticicia oligotrophica
analysis tool for the analysis and modification of human and animal milk oligosaccharides Emticicia oligotrophica
synthesis the transfucosylation ability of one of the fucosidase isoform might be of interest for the synthesis of fucosides Emticicia oligotrophica

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Emticicia oligotrophica

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, 47% loss of activity of Eo3066 Emticicia oligotrophica
Mn2+ 1 mM, 39% loss of activity of Eo3066 Emticicia oligotrophica
Zn2+ 1 mM, 42% loss of activity of Eo3066 Emticicia oligotrophica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.75
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.5, 37°C Emticicia oligotrophica
8.41
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.5, 37°C Emticicia oligotrophica
8.63
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.5, 37°C Emticicia oligotrophica

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 50% increase in activity Emticicia oligotrophica
Ca2+ 1 mM, 133% increase in activity Emticicia oligotrophica
Mg2+ 1 mM, 76% increase in activity Emticicia oligotrophica
Mn2+ 1 mM, 56% increase in activity Emticicia oligotrophica
Mn2+ 1 mM, 64% increase in activity Emticicia oligotrophica
Zn2+ 1 mM, 99% increase in activity Emticicia oligotrophica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 SDS-PAGE Emticicia oligotrophica
55000
-
 SDS-PAGE Emticicia oligotrophica
59000
-
 SDS-PAGE Emticicia oligotrophica
100000
-
 more than 100000 Da, non-denaturing PAGE Emticicia oligotrophica

Organism

Organism UniProt Comment Textmining
Emticicia oligotrophica
-
-
-
Emticicia oligotrophica DSM 17448
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Emticicia oligotrophica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
 substrate: 4-nitrophenyl-alpha-L-fucopyranoside, pH 6.5, 37°C Emticicia oligotrophica
0.34
-
 substrate: 4-nitrophenyl-alpha-L-fucopyranoside, pH 6.5, 37°C Emticicia oligotrophica
2.22
-
 substrate: 4-nitrophenyl-alpha-L-fucopyranoside, pH 6.5, 37°C Emticicia oligotrophica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-fucosyllactose + H2O cleavage of alpha-1,3 bond, low efficiency Emticicia oligotrophica L-fucose +
-
 ?
3-fucosyllactose + H2O cleavage of alpha-1,3 bond, low efficiency Emticicia oligotrophica DSM 17448 L-fucose +
-
 ?
3-fucosyllactose + H2O cleavage of alpha-1,3 bond Emticicia oligotrophica L-fucose + ?
-
 ?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Emticicia oligotrophica 4-nitrophenol + L-fucose
-
 ?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Emticicia oligotrophica DSM 17448 4-nitrophenol + L-fucose
-
 ?
lacto-N-difucohexaose I + H2O cleavage of alpha-1,4 bond Emticicia oligotrophica L-fucose + ?
-
 ?
lacto-N-difucohexaose II + H2O cleavage of alpha-1,4 bond Emticicia oligotrophica L-fucose + ?
-
 ?
lacto-N-fucopentaose + H2O cleavage of alpha-1,3 bond Emticicia oligotrophica L-fucose +
-
 ?
lacto-N-fucopentaose + H2O cleavage of alpha-1,3 bond, low efficiency Emticicia oligotrophica L-fucose +
-
 ?
mono-sialylated-galactosylated biantennary N-glycan-core-substituted with alpha-(1->6)-linked fucose + H2O
-
 Emticicia oligotrophica L-fucose + ?
-
 ?
additional information the enzyme can only cleave alpha1,3-linked fucose residues from 3-fucosyllactose and lacto-N-fucopentaose III with low efficiency Emticicia oligotrophica ?
-
 ?
additional information the enzyme hydrolyzes alpha-(1->6)-linked fucose exclusively Emticicia oligotrophica ?
-
 ?
additional information the enzyme showed good hydrolytic activities towards various alpha1,3- and alpha1,4-bearing fucosyloligosaccharide glycan structures, whereas no defucosylation is observed with the tested alpha1,2- and alpha1,6-fucose containing oligosaccharides Emticicia oligotrophica ?
-
 ?
additional information the enzyme can only cleave alpha1,3-linked fucose residues from 3-fucosyllactose and lacto-N-fucopentaose III with low efficiency Emticicia oligotrophica DSM 17448 ?
-
 ?
additional information the enzyme hydrolyzes alpha-(1->6)-linked fucose exclusively Emticicia oligotrophica DSM 17448 ?
-
 ?
additional information the enzyme showed good hydrolytic activities towards various alpha1,3- and alpha1,4-bearing fucosyloligosaccharide glycan structures, whereas no defucosylation is observed with the tested alpha1,2- and alpha1,6-fucose containing oligosaccharides Emticicia oligotrophica DSM 17448 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Emticicia oligotrophica
homooligomer x * 52000, SDS-PAGE Emticicia oligotrophica
homooligomer x * 59000, SDS-PAGE Emticicia oligotrophica

Synonyms

Synonyms Comment Organism
Eo0918
-
 Emticicia oligotrophica
Eo3066
-
 Emticicia oligotrophica
Eo3812
-
 Emticicia oligotrophica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Emticicia oligotrophica
37
-
 assay at Emticicia oligotrophica
42
-
-
 Emticicia oligotrophica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 42 5°C: about 55% of maximal activity, 42°C: about 50% of maximal activity Emticicia oligotrophica
25 50 25°C: about 70% of maximal activity, 50°C: about 30% of maximal activity Emticicia oligotrophica
30 50 30°C: about 40% of maximal activity, 50°C: about 70% of maximal activity Emticicia oligotrophica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
 activity decreases rapidly above 42°C Emticicia oligotrophica
60
-
 almost complete inactivation Emticicia oligotrophica
70
-
 almost complete inactivation Emticicia oligotrophica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.5, 37°C Emticicia oligotrophica
3.61
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.5, 37°C Emticicia oligotrophica
3.73
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.5, 37°C Emticicia oligotrophica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Emticicia oligotrophica
6.5
-
-
 Emticicia oligotrophica
6.5
-
 assay at Emticicia oligotrophica
7
-
-
 Emticicia oligotrophica

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 pH 5.5: about 40% of maximal activity, pH 7.0: about 60% of maximal activity Emticicia oligotrophica
5.5 7 pH 5.5: about 60% of maximal activity, pH 7.0: about 70% of maximal activity Emticicia oligotrophica
6 8.5 pH 6.0: about 30% of maximal activity, pH 8.5: about 50% of maximal activity Emticicia oligotrophica