Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | the hydrolytic activity displays a maximum increase of 138% in the presence of 1.1 M NaCl. The hydrolytic activity is higher when using CaCl2 compared to NaCl. In the synthesis of fucosylated oligosaccharides using 4-nitrophenyl-fucose as donor substrate and lactose as acceptor, the presence of 1.1 M CaCl2 favors the rate of transfucosylation, and improves the yield of synthesis duplicating and triplicating it with lactose concentrations of 58 mM and 146 mM, respectively. CaCl2 does not significatively affect hydrolysis rate in these reactions. The combination of the activating effect of CaCl2, the decrement in water activity and lactose concentration has a synergistic effect favoring the synthesis of fucosylated oligosaccharides | Thermotoga maritima | |
NaCl | the hydrolytic activity displays a maximum increase of 67% in the presence of 0.8 M NaCl | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
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Thermotoga maritima | - |
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Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-L-fucoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + alpha-L-fucose | - |
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additional information | in the synthesis of fucosylated oligosaccharides using 4-nitrophenyl-fucose as donor substrate and lactose as acceptor, the presence of 1.1 M CaCl2 favors the rate of transfucosylation, and improves the yield of synthesis duplicating and triplicating it with lactose concentrations of 58 mM and 146 mM, respectively. CaCl2 does not significatively affect hydrolysis rate in these reactions. The combination of the activating effect of CaCl2, the decrement in water activity and lactose concentration has a synergistic effect favoring the synthesis of fucosylated oligosaccharides | Thermotoga maritima | ? | - |
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