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Literature summary for 3.2.1.51 extracted from

  • Cobucci-Ponzano, B.; Conte, F.; Benelli, D.; Londei, P.; Flagiello, A.; Monti, M.; Pucci, P.; Rossi, M.; Moracci, M.
    The gene of an archaeal alpha-L-fucosidase is expressed by translational frameshifting (2006), Nucleic Acids Res., 34, 4258-4268.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.51

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutants as GST fusions expressed in Escherichia coli strain BL21(RB791) Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
additional information slippery sequence in fucA1 A-AAA-AAT mutated in A-AAG-AAG-T (mutant fucA1B), shows reduced shifting efficiency. Sequence A-AAA-AAT mutated into A-AAG-AAT (single mutant fucA1sm) and C-AAGAAC (triple mutant fucA1tm). Mutant fucA1sm canceles the frameshifting site A and, in the same time, enhances the frameshifting efficiency of site B. In mutant fucA1tm abolished frameshifting Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0287
-
 4-nitrophenyl-alpha-L-fucoside wild-type and mutant fucA1B Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38100
-
 originates from the translational termination of the ribosome at the OCH codon of the fucA1 N-terminal ORF, Western blot analysis Saccharolobus solfataricus
78700
-
 originates from frameshifting in either site A or B of fucA1, Western blot analysis Saccharolobus solfataricus
97000
-
 Western blot analysis Saccharolobus solfataricus
508000
-
 mutant fucA1B, gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by GST purification system, mutant fucA1B purified up to 95% Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-L-fucoside + H2O
-
 Saccharolobus solfataricus alpha-L-fucopyranose + 4-nitrophenol
-
 ?
additional information 4-nitrophenyl-alpha-L-arabinoside, 4-nitrophenyl-alpha-L-rhamnoside, 4-nitrophenyl-alpha-D-glucoside, 4-nitrophenyl-alpha-D-xyloside, 4-nitrophenyl-alpha-D-galactoside and 4-nitrophenyl-alpha-D-mannoside are not substrates for wild-type and mutant fucA1B Saccharolobus solfataricus ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha-fuc
-
 Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
137
-
 4-nitrophenyl-alpha-L-fucoside mutant fucA1B Saccharolobus solfataricus
287
-
 4-nitrophenyl-alpha-L-fucoside wild-type Saccharolobus solfataricus