Literature summary for 3.2.1.51 extracted from

Sulzenbacher, G.; Bignon, C.; Nishimura, T.; Tarling, C.A.; Withers, S.G.; Henrissat, B.; Bourne, Y.
Crystal structure of Thermotoga maritima alpha-L-fucosidase. Insights into the catalytic mechanism and the molecular basis for fucosidosis (2004), J. Biol. Chem., 279, 13119-13128.

Search for more literature for 3.2.1.51

Application

Application	Comment	Organism
medicine	deficiency in enzyme activity is associated with fucosidosis	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
the crystal structure of enzyme is determined at 2.5 A resolution by the MAD method	Thermotoga maritima

Protein Variants

Protein Variants	Comment	Organism
E266A	the mutant exhibit very low hydrolytic activity with p-nitrophenyl alpha-L-fucoside	Thermotoga maritima
E66A	the mutant retains ability to catalyse the hydrolysis of p-nitrophenyl alpha-L-fucoside and fucosyl fluorid, the Km value is increase dramatically	Thermotoga maritima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	p-nitrophenyl alpha-L-fucoside	pH 5.0, 37°C, wild-type enzyme	Thermotoga maritima
0.075	-	alpha-L-fucosyl fluoride	pH 5.0, 25°C, mutant E266A	Thermotoga maritima
0.15	-	alpha-L-fucosyl fluoride	pH 5.0, 25°C, wild-type enzyme	Thermotoga maritima
2.1	-	p-nitrophenyl alpha-L-fucoside	pH 5.0, 37°C, mutant E66A	Thermotoga maritima
2.5	-	alpha-L-fucosyl fluoride	pH 5.0, 25°C, mutant E66A	Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
300000	-	gel filtration	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-L-fucosyl fluoride + H2O	-	Thermotoga maritima	alpha-L-fucose + fluoride	-	?
p-nitrophenyl alpha-L-fucoside + H2O	-	Thermotoga maritima	p-nitrophenol + alpha-L-fucose	-	?

Subunits

Subunits	Comment	Organism
hexamer	hexamer of two trimers	Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.13	-	alpha-L-fucosyl fluoride	pH 5.0, 25°C, mutant E266A	Thermotoga maritima
1.3	-	p-nitrophenyl alpha-L-fucoside	pH 5.0, 37°C, mutant E66A	Thermotoga maritima
1.4	-	alpha-L-fucosyl fluoride	pH 5.0, 25°C, mutant E66A	Thermotoga maritima
7.6	-	alpha-L-fucosyl fluoride	pH 5.0, 25°C, wild-type enzyme	Thermotoga maritima
14.3	-	p-nitrophenyl alpha-L-fucoside	pH 5.0, 37°C, wild-type enzyme	Thermotoga maritima

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Thermotoga maritima

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)