Literature summary for 3.2.1.51 extracted from

Berteau, O.; McCort, I.; Goasdoue, N.; Tissot, B.; Daniel, R.
Characterization of a new alpha-L-fucosidase isolated from the marine mollusk Pecten maximus that catalyzes the hydrolysis of alpha-L-fucose from algal fucoidan (Ascophyllum nodosum) (2002), Glycobiology, 12, 273-282.

Search for more literature for 3.2.1.51

Activating Compound

Activating Compound	Comment	Organism	Structure
EDTA	-	Pecten maximus
additional information	the enzyme activity is increases with the ionic strength from 10 mM to 200 mM	Pecten maximus

Inhibitors

Inhibitors	Comment	Organism	Structure
1-deoxyfuconojirimycin	-	Pecten maximus
1-deoxygalactonojirimycin	-	Pecten maximus
1-deoxymannojirimycin	-	Pecten maximus
1-deoxynojirimycin	-	Pecten maximus
6-N-acetylamino-deoxymannojirimycin	-	Pecten maximus
Cu2+	-	Pecten maximus
Hg2+	-	Pecten maximus
L-fucosamine	-	Pecten maximus
L-fucose	-	Pecten maximus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.65	-	p-nitrophenyl alpha-L-fucopyranoside	pH 4, 60°C	Pecten maximus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	150% activity at 1 mM	Pecten maximus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	4 * 50000, SDS-PAGE	Pecten maximus
200000	-	gel filtration	Pecten maximus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Pecten maximus	the enzyme is able to catalyse the hydrolysis of fucosyl units of Ascophyllum nodosum fucoidan	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Pecten maximus	-	marine mollusk	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pecten maximus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
86	-	-	Pecten maximus

Storage Stability

Storage Stability	Organism
30°C, 30% loss of activity after 40 days	Pecten maximus
60°C, 40% loss of activity after 1 day	Pecten maximus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme is able to catalyse the hydrolysis of fucosyl units of Ascophyllum nodosum fucoidan	Pecten maximus	?	-	?
p-nitrophenyl alpha-L-fucopyranoside + H2O	-	Pecten maximus	p-nitrophenol + alpha-L-fucopyranose	anomeric configuration of the produced fucopyranse	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 50000, SDS-PAGE	Pecten maximus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Pecten maximus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	40% of activity remains after 1 day	Pecten maximus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	-	Pecten maximus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3	5.5	at pH 3.0 and pH 5.5: more than 50% of the fucosidase activity	Pecten maximus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000026	-	1-deoxyfuconojirimycin	pH 4, 60°C	Pecten maximus
0.034	-	1-deoxymannojirimycin	pH 4, 60°C	Pecten maximus
0.055	-	6-N-acetylamino-deoxymannojirimycin	pH 4, 60°C	Pecten maximus
0.24	-	L-fucose	pH 4, 60°C	Pecten maximus
0.316	-	L-fucosamine	pH 4, 60°C	Pecten maximus
1	-	1-deoxynojirimycin	pH 4, 60°C	Pecten maximus
1	-	1-deoxygalactonojirimycin	pH 4, 60°C	Pecten maximus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Pecten maximus	isoelectric focusing	-	6

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Release 2023.1 (January 2023)