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Literature summary for 3.2.1.51 extracted from

  • Cobucci-Ponzano, B.; Trincone, A.; Giordano, A.; Rossi, M.; Moracci, M.
    Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Sulfolobus solfataricus via chemical rescue of an inactive mutant (2003), Biochemistry, 42, 9525-9531.
    View publication on PubMed
Search for more literature for 3.2.1.51

Protein Variants

Protein Variants Comment Organism
D242G azide activates the mutant, while it does not activate the wild-type enzyme, formate activates mutant higher than wild-type enzyme, the reaction catalysed by the mutant depends less on temperature Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
an alpha-L-fucoside + H2O = L-fucose + an alcohol mechanism Saccharolobus solfataricus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl alpha-L-fucoside + H2O
-
 Saccharolobus solfataricus p-nitrophenol + alpha-L-fucose
-
 ?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 comparison of temperature range depending of wild-type and mutant enzyme, the reaction catalyzed by the mutant D242G depends less on temperature Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.24
-
 p-nitrophenyl alpha-L-fucoside pH 6.5, 65°C, D242G mutant Saccharolobus solfataricus
245
-
 p-nitrophenyl alpha-L-fucoside pH 6.5, 65°C, wild-type enzyme Saccharolobus solfataricus