Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 13% activation at 0.2 mM | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT | Homo sapiens | |
Hg2+ | complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
4-methylumbelliferyl-alpha-L-fucopyranoside | - |
Homo sapiens | |
0.43 | - |
4-nitrophenyl-alpha-L-fucopyranoside | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 22% stimulation at 5 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
230000 | - |
sedimentation equilibrium centrifugation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | Homo sapiens | low activity | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | Homo sapiens | lacto-N-fucopentaose I | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | Homo sapiens | 2'-fucosyllactose | alpha-L-fucose + lactose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 1% carbohydrate content per molecule | Homo sapiens |
Purification (Comment) | Organism |
---|---|
to homogeneity, 2step affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.2 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, fully active after 29 days, 0-4°C, 10 mM Na2HPO4, pH 5.5, 0.02% NaN3, w/v, 100 mM L-fucose, 163 days | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O | - |
Homo sapiens | 4-methylumbelliferone + alpha-L-fucose | - |
? | |
4-nitrophenyl-alpha-L-fucopyranoside + H2O | - |
Homo sapiens | 4-nitrophenol + alpha-L-fucose | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | low activity | Homo sapiens | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | lacto-N-fucopentaose I | Homo sapiens | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | 2'-fucosyllactose | Homo sapiens | alpha-L-fucose + lactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | subunits of 50.1 kDa each, SDS-PAGE | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
gradual loss of activity after 6 min for the purified enzyme, in crude state: stable for 4 h | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | - |
one of two pH-optima | Homo sapiens |
6.5 | - |
second pH-optimum | Homo sapiens |