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Literature summary for 3.2.1.51 extracted from
- Human alpha-fucosidase. Purification and properties (1976), Biochim. Biophys. Acta, 429, 527-537.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | increases acitivity up to 100% | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
4-methylumbelliferyl-alpha-L-fucopyranoside | - |
Homo sapiens |  |
| 0.38 | - |
4-nitrophenyl-alpha-L-fucopyranoside | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
4 * 50000, gel filtration | Homo sapiens |
| 200000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, affinity chromatography, isoelectric focusing, 2 forms, alpha-L-fucosidase A and B | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
| serum | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.8 | - |
alpha-L-fucosidase B | Homo sapiens |
| 3.81 | - |
alpha-L-fucosidase A | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-L-fucopyranoside + H2O | - |
Homo sapiens | 4-methylumbelliferone + alpha-L-fucose | - |
? | |
| 4-nitrophenyl-alpha-L-fucopyranoside + H2O | - |
Homo sapiens | 4-nitrophenol + alpha-L-fucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 50000, gel filtration | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 51 | - |
rapid inactivation for alpha-L-fucosidase A, form B more heat-stable, stabilizing effect of bovine serum albumin, addition of 0.5 M potassium thiocyanate leads to more heat sensitivity for both forms | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
for 4-nitrophenyl-alpha-L-fucopyranoside | Homo sapiens |
| 6 | - |
for 4-methylumbelliferyl-alpha-L-fucopyranoside | Homo sapiens |
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Release 2023.1 (January 2023)
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