General Stability | Organism |
---|---|
stabilizing effect by addition of 0.05 mM/ml bovine serum albumin, 1 mM 2-mercaptoethanol, 1 mM MgSO4, high protein concentrations required | Rattus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-L-fucose | competitive inhibition, Ki: 1.6 mM | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
4-nitrophenyl-alpha-L-fucopyranoside | - |
Rattus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Rattus sp. | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
4 * 55000, SDS-PAGE | Rattus sp. |
217000 | - |
sedimentation equilibrium centrifugation | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycopeptide + H2O | Rattus sp. | not active on native glycoproteins | alpha-L-fucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | residues per tetramer: fucose 3.5, mannose 32, galactose 8: glucose 9, glucosamine 32, sialic acid 8 | Rattus sp. |
Purification (Comment) | Organism |
---|---|
to homogeneity, affinity chromatography | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27 | 28 | - |
Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-L-fucopyranoside + H2O | can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages | Rattus sp. | 4-nitrophenol + alpha-L-fucose | - |
? | |
glycopeptide + H2O | not active on native glycoproteins | Rattus sp. | alpha-L-fucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 55000, SDS-PAGE | Rattus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.7 | 5.9 | - |
Rattus sp. |