Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.51 extracted from

  • Grove, D.S.; Serif, G.S.
    Porcine thyroid fucosidase (1981), Biochim. Biophys. Acta, 662, 246-255.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate complete inactivation at 22 mM Sus scrofa
4-mercuribenzoate complete inhibition at 0.25 mM Sus scrofa
alpha-L-fucose competitive inhibition, Ki: 0.13 mM Sus scrofa
D-arabinose competitive inhibition, Ki: 50 mM Sus scrofa
D-galactose competitive inhibition, Ki: 1.7 mM Sus scrofa
L-rhamnose competitive inhibition, Ki: 140 mM Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
4 * 55000, SDS-PAGE Sus scrofa
192000
-
gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-L-fucopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol + H2O Sus scrofa
-
alpha-L-fucose + beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O Sus scrofa lacto-N-fucopentaose I alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
?
lacto-N-fucopentaose II + H2O Sus scrofa
-
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
?
thyroglobulin + H2O Sus scrofa hydrolysis only after pronase or neuraminidase treatment alpha-L-fucose + ?
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, affinity chromatography Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
thyroid gland
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.8
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
Sus scrofa 4-methylumbelliferone + alpha-L-fucose
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
Sus scrofa 4-nitrophenol + alpha-L-fucose
-
?
alpha-L-fucopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol + H2O
-
Sus scrofa alpha-L-fucose + beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaose I Sus scrofa alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
?
lacto-N-fucopentaose II + H2O
-
Sus scrofa alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
?
submaxilary mucin trisaccharide + H2O
-
Sus scrofa alpha-L-fucose + disaccharide
-
?
thyroglobulin + H2O hydrolysis only after pronase or neuraminidase treatment Sus scrofa alpha-L-fucose + ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 55000, SDS-PAGE Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1
-
-
Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
140
-
L-rhamnose competitive inhibition Sus scrofa