Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate | complete inactivation at 22 mM | Sus scrofa | |
4-mercuribenzoate | complete inhibition at 0.25 mM | Sus scrofa | |
alpha-L-fucose | competitive inhibition, Ki: 0.13 mM | Sus scrofa | |
D-arabinose | competitive inhibition, Ki: 50 mM | Sus scrofa | |
D-galactose | competitive inhibition, Ki: 1.7 mM | Sus scrofa | |
L-rhamnose | competitive inhibition, Ki: 140 mM | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
4-nitrophenyl-alpha-L-fucopyranoside | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
4 * 55000, SDS-PAGE | Sus scrofa |
192000 | - |
gel filtration | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-L-fucopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol + H2O | Sus scrofa | - |
alpha-L-fucose + beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | Sus scrofa | lacto-N-fucopentaose I | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
lacto-N-fucopentaose II + H2O | Sus scrofa | - |
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
thyroglobulin + H2O | Sus scrofa | hydrolysis only after pronase or neuraminidase treatment | alpha-L-fucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Sus scrofa |
Purification (Comment) | Organism |
---|---|
to homogeneity, affinity chromatography | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thyroid gland | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O | - |
Sus scrofa | 4-methylumbelliferone + alpha-L-fucose | - |
? | |
4-nitrophenyl-alpha-L-fucopyranoside + H2O | - |
Sus scrofa | 4-nitrophenol + alpha-L-fucose | - |
? | |
alpha-L-fucopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol + H2O | - |
Sus scrofa | alpha-L-fucose + beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | lacto-N-fucopentaose I | Sus scrofa | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
lacto-N-fucopentaose II + H2O | - |
Sus scrofa | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
submaxilary mucin trisaccharide + H2O | - |
Sus scrofa | alpha-L-fucose + disaccharide | - |
? | |
thyroglobulin + H2O | hydrolysis only after pronase or neuraminidase treatment | Sus scrofa | alpha-L-fucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 55000, SDS-PAGE | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.1 | - |
- |
Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
140 | - |
L-rhamnose | competitive inhibition | Sus scrofa |