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Literature summary for 3.2.1.51 extracted from
- Induction and purification of alpha-L-fucosidase from Fusarium oxysporum (1986), Agric. Biol. Chem., 50, 1689-1695.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-L-fucose | 90% competitive inhibition at 5 mM, Ki: 0.12 mM | Fusarium oxysporum |  |
| Hg2+ | complete inhibition at 1 mM | Fusarium oxysporum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.48 | - |
4-nitrophenyl-alpha-L-fucopyranoside | - |
Fusarium oxysporum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | induction of enzyme synthesis by addition of L-fucose to the medium | Fusarium oxysporum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
gel filtration | Fusarium oxysporum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fucoidan + H2O | Fusarium oxysporum | - |
alpha-L-fucose + ? | - |
? | |
| gastric mucin + H2O | Fusarium oxysporum | porcine | alpha-L-fucose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium oxysporum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Fusarium oxysporum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneous, chromatography steps | Fusarium oxysporum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Fusarium oxysporum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 110 | - |
- |
Fusarium oxysporum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl-alpha-L-fucopyranoside + H2O | - |
Fusarium oxysporum | 4-nitrophenol + alpha-L-fucose | - |
? | |
| fucoidan + H2O | - |
Fusarium oxysporum | alpha-L-fucose + ? | - |
? | |
| gastric mucin + H2O | porcine | Fusarium oxysporum | alpha-L-fucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 75000, SDS-PAGE | Fusarium oxysporum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Fusarium oxysporum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable below | Fusarium oxysporum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6 | optimal activity within | Fusarium oxysporum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | stable within | Fusarium oxysporum |
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